P2Y12R-Dependent Translocation Mechanisms Gate the Changing Microglial Landscape

Ukpong B. Eyo, Mingshu Mo, Min Hee Yi, Madhuvika Murugan, Junting Liu, Rohan Yarlagadda, David J. Margolis, Pingyi Xu, LongJun Wu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Microglia are an exquisitely tiled and self-contained population in the CNS that do not receive contributions from circulating monocytes in the periphery. While microglia are long-lived cells, the extent to which their cell bodies are fixed and the molecular mechanisms by which the microglial landscape is regulated have not been determined. Using chronic in vivo two-photon imaging to follow the microglial population in young adult mice, we document a daily rearrangement of the microglial landscape. Furthermore, we show that the microglial landscape can be modulated by severe seizures, acute injury, and sensory deprivation. Finally, we demonstrate a critical role for microglial P2Y12Rs in regulating the microglial landscape through cellular translocation independent of proliferation. These findings suggest that microglial patrol the CNS through both process motility and soma translocation. Using a long-term high-resolution in vivo imaging approach, Eyo et al. show that the physical positions of brain microglia change daily and that these changes increase following certain experimental manipulations. The mechanism underlying these changes involves cell translocation controlled by microglial-specific P2Y12 receptors.

Original languageEnglish (US)
JournalCell Reports
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Microglia
Sensory Deprivation
Imaging techniques
Carisoprodol
Photons
Population
Monocytes
Young Adult
Brain
Seizures
Cells
Wounds and Injuries

Keywords

  • epilepsy
  • microglia
  • microglial landscape
  • P2Y12
  • seizures
  • two photon chronic imaging
  • whisker trimming

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

P2Y12R-Dependent Translocation Mechanisms Gate the Changing Microglial Landscape. / Eyo, Ukpong B.; Mo, Mingshu; Yi, Min Hee; Murugan, Madhuvika; Liu, Junting; Yarlagadda, Rohan; Margolis, David J.; Xu, Pingyi; Wu, LongJun.

In: Cell Reports, 01.01.2018.

Research output: Contribution to journalArticle

Eyo, Ukpong B. ; Mo, Mingshu ; Yi, Min Hee ; Murugan, Madhuvika ; Liu, Junting ; Yarlagadda, Rohan ; Margolis, David J. ; Xu, Pingyi ; Wu, LongJun. / P2Y12R-Dependent Translocation Mechanisms Gate the Changing Microglial Landscape. In: Cell Reports. 2018.
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