P2X 3 receptor subunit messenger RNA expression in the female mouse bladder after oophorectomy with or without estrogen replacement

Michael E. Carley, William Arthur Cliby, Thomas C. Spelsberg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

OBJECTIVE: This investigation was undertaken to determine whether the sensory neuron adenosine triphosphate receptor subunit (P2X 3) messenger RNA expression is altered in female mouse bladders after surgical oophorectomy with or without estrogen replacement. STUDY DESIGN: The mean relative concentrations of the P2X 3 receptor in 30 female mouse bladders (10 sham operated, 10 oophorectomized, and 10 oophorectomized with estrogen replacement) were determined with quantitative reverse transcription-polymerase chain reaction analysis. RESULTS: P2X 3 expression increased after surgical oophorectomy (0.91 ± 0.16 vs 1.04 ± 0.11, P = .048). However, P2X 3 expression after oophorectomy and immediate estrogen replacement did not differ from that of oophorectomy alone (1.11 ± 0.15 vs 1.04 ± 0.11, P = .206). CONCLUSIONS: The P2X 3 sensory neuron receptor messenger RNA expression is increased after oophorectomy but is not influenced by subsequent estrogen replacement. This has clinical significance because sensory neuron receptors may be associated with certain forms of bladder dysfunction.

Original languageEnglish (US)
Pages (from-to)103-106
Number of pages4
JournalAmerican Journal of Obstetrics and Gynecology
Volume187
Issue number1
DOIs
StatePublished - 2002

Fingerprint

Estrogen Replacement Therapy
Ovariectomy
Sensory Receptor Cells
Urinary Bladder
Messenger RNA
Purinergic P1 Receptors
Reverse Transcription
Adenosine Triphosphate
Polymerase Chain Reaction

Keywords

  • Bladder
  • Estrogen
  • Mouse
  • P2X receptor
  • Urinary incontinence

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

P2X 3 receptor subunit messenger RNA expression in the female mouse bladder after oophorectomy with or without estrogen replacement. / Carley, Michael E.; Cliby, William Arthur; Spelsberg, Thomas C.

In: American Journal of Obstetrics and Gynecology, Vol. 187, No. 1, 2002, p. 103-106.

Research output: Contribution to journalArticle

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