Oxygen gradients in CHO cells

Measurement and characterization by electron spin resonance

James Glockner, H. M. Swartz, M. A. Pals

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The concentration of oxygen within cells is important in mnay physiological and pathological processes, but such oxygen-dependent processes are generally studied as a function of the concentration of extracellular oxygen, due to a lack of suitable methods. Using a newly developed technique based on ESR spectroscopy, we show that respiration stimulation of a cell suspension can result in a significant difference between average intracellular and extracellular concentrations of oxygen. These results indicate that studies of oxygen-dependent phenomena in cells may require measurement of intracellular oxygen concentrations and imply that there are mechanisms in cells that restrict the free diffusion of oxygen.

Original languageEnglish (US)
Pages (from-to)505-511
Number of pages7
JournalJournal of Cellular Physiology
Volume140
Issue number3
StatePublished - 1989
Externally publishedYes

Fingerprint

CHO Cells
Electron Spin Resonance Spectroscopy
Paramagnetic resonance
Oxygen
Physiological Phenomena
Pathologic Processes
Spectrum Analysis
Suspensions
Respiration
Spectroscopy

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Oxygen gradients in CHO cells : Measurement and characterization by electron spin resonance. / Glockner, James; Swartz, H. M.; Pals, M. A.

In: Journal of Cellular Physiology, Vol. 140, No. 3, 1989, p. 505-511.

Research output: Contribution to journalArticle

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