TY - JOUR
T1 - Oximetry in cells and tissues using a nitroxide-liposome system
AU - Chan, Hsiao Chang
AU - Glockner, James F.
AU - Swartz, Harold M.
N1 - Funding Information:
This work was supported by NIH grant GM 34250, and used the facilities of the ESR center at the University of Illinois which is supported by NIH grant RR 01811. H.-C.C. and .I.F.G. received support from NIH training grant CA 09067.
PY - 1989/11/20
Y1 - 1989/11/20
N2 - In order to avoid the complication of reduction of nitroxides in biological media during oxygen measurements, liposomes containing a water-soluble nitroxide, 2,2,6,6-tetramethylpiperidine-N-oxyl-4-trimethylammonium (Catl), were used in studies of oxygen consumption by thymus-bone-marrow cells. The superhyperfine structure of Cat1 contained in liposomes was found to be sensitive to oxygen concentration in a fashion similar to that of free Cat1. Measurements of cellular respiration using Cat1 contained in liposomes agreed well with the results obtained using free Cat1. Using this nitroxide-liposome system, the respiration of liver slices was measured successfully, whereas such measurements using free Cat1 were complicated by rapid reduction of the nitroxide. This nitroxide-liposome system also could be used in conjunction with a membrane permeable nitroxide and an extracellular broadening agent to measure intracellular and extracellular oxygen concentrations simultaneously.
AB - In order to avoid the complication of reduction of nitroxides in biological media during oxygen measurements, liposomes containing a water-soluble nitroxide, 2,2,6,6-tetramethylpiperidine-N-oxyl-4-trimethylammonium (Catl), were used in studies of oxygen consumption by thymus-bone-marrow cells. The superhyperfine structure of Cat1 contained in liposomes was found to be sensitive to oxygen concentration in a fashion similar to that of free Cat1. Measurements of cellular respiration using Cat1 contained in liposomes agreed well with the results obtained using free Cat1. Using this nitroxide-liposome system, the respiration of liver slices was measured successfully, whereas such measurements using free Cat1 were complicated by rapid reduction of the nitroxide. This nitroxide-liposome system also could be used in conjunction with a membrane permeable nitroxide and an extracellular broadening agent to measure intracellular and extracellular oxygen concentrations simultaneously.
KW - Cat1
KW - ESR
KW - Liposome
KW - Oxygen concentration
KW - Superhyperfine structure
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U2 - 10.1016/0167-4889(89)90026-8
DO - 10.1016/0167-4889(89)90026-8
M3 - Article
C2 - 2554973
AN - SCOPUS:0024470587
SN - 0167-4889
VL - 1014
SP - 141
EP - 144
JO - BBA - Molecular Cell Research
JF - BBA - Molecular Cell Research
IS - 2
ER -