Oximetry in cells and tissues using a nitroxide-liposome system

Hsiao Chang Chan, James F. Glockner, Harold M. Swartz

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In order to avoid the complication of reduction of nitroxides in biological media during oxygen measurements, liposomes containing a water-soluble nitroxide, 2,2,6,6-tetramethylpiperidine-N-oxyl-4-trimethylammonium (Catl), were used in studies of oxygen consumption by thymus-bone-marrow cells. The superhyperfine structure of Cat1 contained in liposomes was found to be sensitive to oxygen concentration in a fashion similar to that of free Cat1. Measurements of cellular respiration using Cat1 contained in liposomes agreed well with the results obtained using free Cat1. Using this nitroxide-liposome system, the respiration of liver slices was measured successfully, whereas such measurements using free Cat1 were complicated by rapid reduction of the nitroxide. This nitroxide-liposome system also could be used in conjunction with a membrane permeable nitroxide and an extracellular broadening agent to measure intracellular and extracellular oxygen concentrations simultaneously.

Original languageEnglish (US)
Pages (from-to)141-144
Number of pages4
JournalBBA - Molecular Cell Research
Volume1014
Issue number2
DOIs
StatePublished - Nov 20 1989

Keywords

  • Cat1
  • ESR
  • Liposome
  • Oxygen concentration
  • Superhyperfine structure

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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