Oxidative stress-induced afterdepolarizations and protein kinase C signaling

Yu Dong Fei, Wei Li, Jian Wen Hou, Kai Guo, Xiao Meng Chen, Yi He Chen, Qian Wang, Xiaolei H Xu, Yue Peng Wang, Yi Gang Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Hydrogen peroxide (H2O2)-induced oxidative stress has been demonstrated to induce afterdepolarizations and triggered activities in isolated myocytes, but the underlying mechanisms remain not fully understood. We aimed to explore whether protein kinase C (PKC) activation plays an important role in oxidative stress-induced afterdepolarizations. Methods: Action potentials and ion currents of isolated rabbit cardiomyocytes were recorded using the patch clamp technique. H2O2 (1 mM) was perfused to induce oxidative stress and the specific classical PKC inhibitor, Gö 6983 (1 µM), was applied to test the involvement of PKC. Results: H2O2 perfusion prolonged the action potential duration and induced afterdepolarizations. Pretreatment with Gö 6983 prevented the emergence of H2O2-induced afterdepolarizations. Additional application of Gö 6983 with H2O2 effectively suppressed H2O2-induced afterdepolarizations. H2O2 increased the late sodium current (INa,L) (n = 7, p < 0.01) and the L-type calcium current (ICa,L) (n = 5, p < 0.01), which were significantly reversed by Gö 6983 (p < 0.01). H2O2 also increased the transient outward potassium current (Ito) (n = 6, p < 0.05). However, Gö 6983 showed little effect on H2O2-induced enhancement of Ito. Conclusions: H2O2 induced afterdepolarizations via the activation of PKC and the enhancement of ICa,L and INa,L. These results provide evidence of a link between oxidative stress, PKC activation and afterdepolarizations.

Original languageEnglish (US)
Article number688
JournalInternational Journal of Molecular Sciences
Volume18
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Oxidative stress
Protein Kinase C
Oxidative Stress
proteins
Proteins
Chemical activation
activation
Action Potentials
muscle cells
clamps
Protein C Inhibitor
augmentation
rabbits
Clamping devices
Patch-Clamp Techniques
Protein Kinase Inhibitors
Heat-Shock Proteins
hydrogen peroxide
Cardiac Myocytes
Hydrogen peroxide

Keywords

  • Afterdepolarization
  • Arrhythmia
  • Oxidative stress
  • Protein kinase C
  • Triggered activity

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Fei, Y. D., Li, W., Hou, J. W., Guo, K., Chen, X. M., Chen, Y. H., ... Li, Y. G. (2017). Oxidative stress-induced afterdepolarizations and protein kinase C signaling. International Journal of Molecular Sciences, 18(4), [688]. https://doi.org/10.3390/ijms18040688

Oxidative stress-induced afterdepolarizations and protein kinase C signaling. / Fei, Yu Dong; Li, Wei; Hou, Jian Wen; Guo, Kai; Chen, Xiao Meng; Chen, Yi He; Wang, Qian; Xu, Xiaolei H; Wang, Yue Peng; Li, Yi Gang.

In: International Journal of Molecular Sciences, Vol. 18, No. 4, 688, 01.04.2017.

Research output: Contribution to journalArticle

Fei, YD, Li, W, Hou, JW, Guo, K, Chen, XM, Chen, YH, Wang, Q, Xu, XH, Wang, YP & Li, YG 2017, 'Oxidative stress-induced afterdepolarizations and protein kinase C signaling', International Journal of Molecular Sciences, vol. 18, no. 4, 688. https://doi.org/10.3390/ijms18040688
Fei, Yu Dong ; Li, Wei ; Hou, Jian Wen ; Guo, Kai ; Chen, Xiao Meng ; Chen, Yi He ; Wang, Qian ; Xu, Xiaolei H ; Wang, Yue Peng ; Li, Yi Gang. / Oxidative stress-induced afterdepolarizations and protein kinase C signaling. In: International Journal of Molecular Sciences. 2017 ; Vol. 18, No. 4.
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abstract = "Background: Hydrogen peroxide (H2O2)-induced oxidative stress has been demonstrated to induce afterdepolarizations and triggered activities in isolated myocytes, but the underlying mechanisms remain not fully understood. We aimed to explore whether protein kinase C (PKC) activation plays an important role in oxidative stress-induced afterdepolarizations. Methods: Action potentials and ion currents of isolated rabbit cardiomyocytes were recorded using the patch clamp technique. H2O2 (1 mM) was perfused to induce oxidative stress and the specific classical PKC inhibitor, G{\"o} 6983 (1 µM), was applied to test the involvement of PKC. Results: H2O2 perfusion prolonged the action potential duration and induced afterdepolarizations. Pretreatment with G{\"o} 6983 prevented the emergence of H2O2-induced afterdepolarizations. Additional application of G{\"o} 6983 with H2O2 effectively suppressed H2O2-induced afterdepolarizations. H2O2 increased the late sodium current (INa,L) (n = 7, p < 0.01) and the L-type calcium current (ICa,L) (n = 5, p < 0.01), which were significantly reversed by G{\"o} 6983 (p < 0.01). H2O2 also increased the transient outward potassium current (Ito) (n = 6, p < 0.05). However, G{\"o} 6983 showed little effect on H2O2-induced enhancement of Ito. Conclusions: H2O2 induced afterdepolarizations via the activation of PKC and the enhancement of ICa,L and INa,L. These results provide evidence of a link between oxidative stress, PKC activation and afterdepolarizations.",
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AU - Chen, Yi He

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AU - Xu, Xiaolei H

AU - Wang, Yue Peng

AU - Li, Yi Gang

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N2 - Background: Hydrogen peroxide (H2O2)-induced oxidative stress has been demonstrated to induce afterdepolarizations and triggered activities in isolated myocytes, but the underlying mechanisms remain not fully understood. We aimed to explore whether protein kinase C (PKC) activation plays an important role in oxidative stress-induced afterdepolarizations. Methods: Action potentials and ion currents of isolated rabbit cardiomyocytes were recorded using the patch clamp technique. H2O2 (1 mM) was perfused to induce oxidative stress and the specific classical PKC inhibitor, Gö 6983 (1 µM), was applied to test the involvement of PKC. Results: H2O2 perfusion prolonged the action potential duration and induced afterdepolarizations. Pretreatment with Gö 6983 prevented the emergence of H2O2-induced afterdepolarizations. Additional application of Gö 6983 with H2O2 effectively suppressed H2O2-induced afterdepolarizations. H2O2 increased the late sodium current (INa,L) (n = 7, p < 0.01) and the L-type calcium current (ICa,L) (n = 5, p < 0.01), which were significantly reversed by Gö 6983 (p < 0.01). H2O2 also increased the transient outward potassium current (Ito) (n = 6, p < 0.05). However, Gö 6983 showed little effect on H2O2-induced enhancement of Ito. Conclusions: H2O2 induced afterdepolarizations via the activation of PKC and the enhancement of ICa,L and INa,L. These results provide evidence of a link between oxidative stress, PKC activation and afterdepolarizations.

AB - Background: Hydrogen peroxide (H2O2)-induced oxidative stress has been demonstrated to induce afterdepolarizations and triggered activities in isolated myocytes, but the underlying mechanisms remain not fully understood. We aimed to explore whether protein kinase C (PKC) activation plays an important role in oxidative stress-induced afterdepolarizations. Methods: Action potentials and ion currents of isolated rabbit cardiomyocytes were recorded using the patch clamp technique. H2O2 (1 mM) was perfused to induce oxidative stress and the specific classical PKC inhibitor, Gö 6983 (1 µM), was applied to test the involvement of PKC. Results: H2O2 perfusion prolonged the action potential duration and induced afterdepolarizations. Pretreatment with Gö 6983 prevented the emergence of H2O2-induced afterdepolarizations. Additional application of Gö 6983 with H2O2 effectively suppressed H2O2-induced afterdepolarizations. H2O2 increased the late sodium current (INa,L) (n = 7, p < 0.01) and the L-type calcium current (ICa,L) (n = 5, p < 0.01), which were significantly reversed by Gö 6983 (p < 0.01). H2O2 also increased the transient outward potassium current (Ito) (n = 6, p < 0.05). However, Gö 6983 showed little effect on H2O2-induced enhancement of Ito. Conclusions: H2O2 induced afterdepolarizations via the activation of PKC and the enhancement of ICa,L and INa,L. These results provide evidence of a link between oxidative stress, PKC activation and afterdepolarizations.

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KW - Protein kinase C

KW - Triggered activity

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