Oxidative protein damage in cells engaged in β-amyloidosis is related to apoE genotype

Bozena Mazur-Kolecka, Janusz Frackowiak, Dagmar Kowal, Jolanta Krzeslowska, Dennis W Dickson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The ε4 allele of apolipoprotein E (apoE) is a risk factor for Alzheimer's disease. The reduced antioxidant defense in ε4 carriers is suggested to contribute to β-amyloidosis. We found that oxidative stress induced by treatment with Fe2+ ions raised more protein carbonyls in vascular smooth muscle cells isolated from human brains with apoE genotype ε4/ε4 than with 3ε/ε3 and ε3/ε4. Antioxidant vitamin E prevented formation of carbonyls but not in cells with genotype ε4/ε4. Treatment with Fe2+ ions induced cellular accumulation of amyloid-β protein (Aβ)-immunoreactive material that co-localized with heme oxygenase, a marker of oxidative stress, and apoE. We hypothesize that the damage caused by oxidation in ε4/ε4 carriers facilitates development of β-amyloidosis.

Original languageEnglish (US)
Pages (from-to)465-468
Number of pages4
JournalNeuroReport
Volume13
Issue number4
StatePublished - Mar 25 2002

Fingerprint

Apolipoprotein E4
Apolipoproteins E
Amyloidosis
Oxidative Stress
Antioxidants
Genotype
Ions
Serum Amyloid A Protein
Heme Oxygenase (Decyclizing)
Vitamin E
Vascular Smooth Muscle
Smooth Muscle Myocytes
Alzheimer Disease
Proteins
Alleles
Brain
1,21-diamino-4,9,13,18-tetraazahenicosane

Keywords

  • β-Amyloid protein
  • ApoE genotype
  • Cell culture
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mazur-Kolecka, B., Frackowiak, J., Kowal, D., Krzeslowska, J., & Dickson, D. W. (2002). Oxidative protein damage in cells engaged in β-amyloidosis is related to apoE genotype. NeuroReport, 13(4), 465-468.

Oxidative protein damage in cells engaged in β-amyloidosis is related to apoE genotype. / Mazur-Kolecka, Bozena; Frackowiak, Janusz; Kowal, Dagmar; Krzeslowska, Jolanta; Dickson, Dennis W.

In: NeuroReport, Vol. 13, No. 4, 25.03.2002, p. 465-468.

Research output: Contribution to journalArticle

Mazur-Kolecka, B, Frackowiak, J, Kowal, D, Krzeslowska, J & Dickson, DW 2002, 'Oxidative protein damage in cells engaged in β-amyloidosis is related to apoE genotype', NeuroReport, vol. 13, no. 4, pp. 465-468.
Mazur-Kolecka B, Frackowiak J, Kowal D, Krzeslowska J, Dickson DW. Oxidative protein damage in cells engaged in β-amyloidosis is related to apoE genotype. NeuroReport. 2002 Mar 25;13(4):465-468.
Mazur-Kolecka, Bozena ; Frackowiak, Janusz ; Kowal, Dagmar ; Krzeslowska, Jolanta ; Dickson, Dennis W. / Oxidative protein damage in cells engaged in β-amyloidosis is related to apoE genotype. In: NeuroReport. 2002 ; Vol. 13, No. 4. pp. 465-468.
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