Overexpression of the TGFβ-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells

Issei Tachibana, Mami Imoto, Philip N. Adjei, Gregory James Gores, Malayannan Subramaniam, Thomas C. Spelsberg, Raul Urrutia

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Members of the TGFβ family of peptides exert antiproliferative effects and induce apoptosis in epithelial cell populations. In the exocrine pancreas, these peptides not only regulate normal cell growth, but alterations in these pathways have been associated with neoplastic transformation. Therefore, the identification of molecules that regulate exocrine pancreatic cell proliferation and apoptotic cell death in response to TGFβ peptides is necessary for a better understanding of normal morphogenesis as well as carcinogenesis of the pancreas. In this study, we have characterized the expression and function in exocrine pancreatic epithelial cells of the TGFβ-inducible early gene (TIEG), a Kruppel-like zinc finger transcription factor encoding gene previously isolated from mesodermally derived osteoblastic cells. We demonstrate that this gene is expressed in both acinar and ductular epithelial cell populations from the exocrine pancreas. In addition, we show that the expression of TIEG is regulated by TGFβ1 as an early response gene in pancreatic epithelial cell lines. Moreover, overexpression of TIEG in the TGFβ-sensitive epithelial cell line PANC1 is sufficient to induce apoptosis. Together, these results support a role for TIEG in linking TGFβ-mediated signaling cascades to the regulation of pancreatic epithelial cell growth.

Original languageEnglish (US)
Pages (from-to)2365-2374
Number of pages10
JournalJournal of Clinical Investigation
Volume99
Issue number10
StatePublished - May 15 1997

Fingerprint

Zinc Fingers
Epithelial Cells
Apoptosis
Genes
Exocrine Pancreas
Peptides
Cell Line
Growth
Morphogenesis
Population
Pancreas
Carcinogenesis
Cell Death
Transcription Factors
Cell Proliferation

Keywords

  • apoptosis
  • pancreas
  • transcription factor
  • transforming growth factor-β
  • zinc finger

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tachibana, I., Imoto, M., Adjei, P. N., Gores, G. J., Subramaniam, M., Spelsberg, T. C., & Urrutia, R. (1997). Overexpression of the TGFβ-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells. Journal of Clinical Investigation, 99(10), 2365-2374.

Overexpression of the TGFβ-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells. / Tachibana, Issei; Imoto, Mami; Adjei, Philip N.; Gores, Gregory James; Subramaniam, Malayannan; Spelsberg, Thomas C.; Urrutia, Raul.

In: Journal of Clinical Investigation, Vol. 99, No. 10, 15.05.1997, p. 2365-2374.

Research output: Contribution to journalArticle

Tachibana, I, Imoto, M, Adjei, PN, Gores, GJ, Subramaniam, M, Spelsberg, TC & Urrutia, R 1997, 'Overexpression of the TGFβ-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells', Journal of Clinical Investigation, vol. 99, no. 10, pp. 2365-2374.
Tachibana, Issei ; Imoto, Mami ; Adjei, Philip N. ; Gores, Gregory James ; Subramaniam, Malayannan ; Spelsberg, Thomas C. ; Urrutia, Raul. / Overexpression of the TGFβ-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells. In: Journal of Clinical Investigation. 1997 ; Vol. 99, No. 10. pp. 2365-2374.
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