Overexpression of the E2 ubiquitin-conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation

Janine H. Van Ree, Karthik B. Jeganathan, Liviu Malureanu, Jan Van Deursen

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The anaphase-promoting complex/cyclosome (APC/C) E3 ubiquitin ligase functions with the E2 ubiquitin-conjugating enzyme UbcH10 in the orderly progression through mitosis by marking key mitotic regulators for destruction by the 26-S proteasome. UbcH10 is overexpressed in many human cancer types and is associated with tumor progression. However, whether UbcH10 overexpression causes tumor formation is unknown. To address this central question and to define the molecular and cellular consequences of UbcH10 overexpression, we generated a series of transgenic mice in which UbcH10 was overexpressed in graded fashion. In this study, we show that UbcH10 overexpression leads to precocious degradation of cyclin B by the APC/C, supernumerary centrioles, lagging chromosomes, and aneuploidy. Importantly, we find that UbcH10 transgenic mice are prone to carcinogen-induced lung tumors and a broad spectrum of spontaneous tumors. Our results identify UbcH10 as a prominent protooncogene that causes whole chromosome instability and tumor formation over a wide gradient of overexpression levels.

Original languageEnglish (US)
Pages (from-to)83-100
Number of pages18
JournalJournal of Cell Biology
Volume188
Issue number1
DOIs
StatePublished - Dec 11 2010

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Ubiquitin-Conjugating Enzymes
Chromosomes
Anaphase-Promoting Complex-Cyclosome
Neoplasms
Transgenic Mice
Centrioles
Cyclin B
Chromosomal Instability
Ubiquitin-Protein Ligases
Aneuploidy
Proteasome Endopeptidase Complex
Mitosis
Carcinogens
Lung

ASJC Scopus subject areas

  • Cell Biology

Cite this

Overexpression of the E2 ubiquitin-conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation. / Van Ree, Janine H.; Jeganathan, Karthik B.; Malureanu, Liviu; Van Deursen, Jan.

In: Journal of Cell Biology, Vol. 188, No. 1, 11.12.2010, p. 83-100.

Research output: Contribution to journalArticle

Van Ree, Janine H. ; Jeganathan, Karthik B. ; Malureanu, Liviu ; Van Deursen, Jan. / Overexpression of the E2 ubiquitin-conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation. In: Journal of Cell Biology. 2010 ; Vol. 188, No. 1. pp. 83-100.
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