Overexpression of Periostin and Lumican in Esophageal squamous cell carcinoma

Manoj Kumar Kashyap, Arivusudar Marimuthu, Suraj Peri, Ghantasala S.Sameer Kumar, Harrys K.C. Jacob, Thottethodi Subrahmanya Keshava Prasad, Riaz Mahmood, K. V.Veerendra Kumar, M. Vijaya Kumar, Stephen J. Meltzer, Elizabeth A. Montgomery, Rekha V. Kumar, Akhilesh Pandey

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

To identify biomarkers for early detection for esophageal squamous cell carcinoma (ESCC), we previously carried out a genome-wide gene expression profiling study using an oligonucleotide microarray platform. This analysis led to identification of several transcripts that were significantly upregulated in ESCC compared to the adjacent normal epithelium. In the current study, we performed immunohistochemical analyses of protein products for two candidates genes identified from the DNA microarray analysis, periostin (POSTN) and lumican (LUM), using tissue microarrays. Increased expression of both periostin and lumican was observed in 100% of 137 different ESCC samples arrayed on tissue microarrays. Increased expression of periostin and lumican was observed in carcinoma as well as in stromal cell in the large majority of cases. These findings suggest that these candidates can be investigated in the sera of ESCC patients using ELISA or multiple reaction monitoring (MRM) type assays to further explore their utility as biomarkers.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalCancers
Volume2
Issue number1
DOIs
StatePublished - Mar 2010

Keywords

  • DNA microarrays
  • Epithelial-mesenchymal transition
  • Extracellular matrix
  • Stroma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Kashyap, M. K., Marimuthu, A., Peri, S., Kumar, G. S. S., Jacob, H. K. C., Prasad, T. S. K., Mahmood, R., Kumar, K. V. V., Kumar, M. V., Meltzer, S. J., Montgomery, E. A., Kumar, R. V., & Pandey, A. (2010). Overexpression of Periostin and Lumican in Esophageal squamous cell carcinoma. Cancers, 2(1), 133-142. https://doi.org/10.3390/cancers2010133