Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer

Deepthi Telikicherla, Jagadeesha Maharudraiah, Harsh Pawar, Arivusudar Marimuthu, Manoj Kumar Kashyap, Y. L. Ramachandra, Juan Carlos Roa, Akhilesh Pandey

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Gene expression profiling studies on breast cancer have generated catalogs of differentially expressed genes. However, many of these genes have not been investigated for their expression at the protein level. It is possible to systematically evaluate such genes in a high-throughput fashion for their overexpression at the protein level using breast cancer tissue microarrays. Our strategy involved integration of information from publicly available repositories of gene expression to prepare a list of genes upregulated at the mRNA level in breast cancer followed by curation of the published literature to identify those genes that were not tested for overexpression at the protein level. We identified Kinesin Associated Protein 3 (KIFAP3) as one such molecule for further validation at the protein level. Immunohistochemical labeling of KIFAP3 using breast cancer tissue microarrays revealed overexpression of KIFAP3 protein in 84% (240/285) of breast cancers indicating the utility of our integrated approach of combining computational analysis with experimental biology.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalJournal of Proteomics and Bioinformatics
Volume5
Issue number5
DOIs
StatePublished - May 2012

Keywords

  • Breast neoplasms
  • Immunohistochemistry
  • Kap3
  • Smap
  • Smggds associated protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Cell Biology

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    Telikicherla, D., Maharudraiah, J., Pawar, H., Marimuthu, A., Kashyap, M. K., Ramachandra, Y. L., Roa, J. C., & Pandey, A. (2012). Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer. Journal of Proteomics and Bioinformatics, 5(5), 122-126. https://doi.org/10.4172/jpb.1000223