Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease

Suzanne E. Wahrle, Hong Jiang, Maia Parsadanian, Jungsu Kim, Aimin Li, Amanda Knoten, Sanjay Jain, Veronica Hirsch-Reinshagen, Cheryl L. Wellington, Kelly R. Bales, Steven M. Paul, David M. Holtzman

Research output: Contribution to journalArticle

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Abstract

APOE genotype is a major genetic risk factor for late-onset Alzheimer disease (AD). ABCA1, a member of the ATP-binding cassette family of active transporters, lipidates apoE in the CNS. Abca1-/- mice have decreased lipid associated with apoE and increased amyloid deposition in several AD mouse models. We hypothesized that mice overexpressing ABCA1 in the brain would have increased lipidation of apoE-containing lipoproteins and decreased amyloid deposition. To address these hypotheses, we created PrP-mAbca1 Tg mice that overexpress mouse Abca1 throughout the brain under the control of the mouse prion promoter. We bred the PrP-mAbca1 mice to the PDAPP AD mouse model, a transgenic line overexpressing a mutant human amyloid precursor protein. PDAPP/Abca1 Tg mice developed a phenotype remarkably similar to that seen in PDAPP/Apoe-/- mice: there was significantly less amyloid β-peptide (Aβ) deposition, a redistribution of Aβ to the hilus of the dentate gyrus in the hippocampus, and an almost complete absence of thioflavine S-positive amyloid plaques. Analyses of CSF from PrP-mAbca1 Tg mice and media conditioned by PrP-mAbca1 Tg primary astrocytes demonstrated increased lipidation of apoE-containing particles. These data support the conclusions that increased ABCA1-mediated lipidation of apoE in the CNS can reduce amyloid burden and that increasing ABCA1 function may have a therapeutic effect on AD.

Original languageEnglish (US)
Pages (from-to)671-682
Number of pages12
JournalJournal of Clinical Investigation
Volume118
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

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Amyloid
Alzheimer Disease
Apolipoproteins E
Parahippocampal Gyrus
Amyloid beta-Protein Precursor
Prions
Dentate Gyrus
Amyloid Plaques
Brain
Therapeutic Uses
Conditioned Culture Medium
Astrocytes
Lipoproteins
Adenosine Triphosphate
Genotype
Phenotype
Lipids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wahrle, S. E., Jiang, H., Parsadanian, M., Kim, J., Li, A., Knoten, A., ... Holtzman, D. M. (2008). Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease. Journal of Clinical Investigation, 118(2), 671-682. https://doi.org/10.1172/JCI33622

Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease. / Wahrle, Suzanne E.; Jiang, Hong; Parsadanian, Maia; Kim, Jungsu; Li, Aimin; Knoten, Amanda; Jain, Sanjay; Hirsch-Reinshagen, Veronica; Wellington, Cheryl L.; Bales, Kelly R.; Paul, Steven M.; Holtzman, David M.

In: Journal of Clinical Investigation, Vol. 118, No. 2, 01.02.2008, p. 671-682.

Research output: Contribution to journalArticle

Wahrle, SE, Jiang, H, Parsadanian, M, Kim, J, Li, A, Knoten, A, Jain, S, Hirsch-Reinshagen, V, Wellington, CL, Bales, KR, Paul, SM & Holtzman, DM 2008, 'Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease', Journal of Clinical Investigation, vol. 118, no. 2, pp. 671-682. https://doi.org/10.1172/JCI33622
Wahrle, Suzanne E. ; Jiang, Hong ; Parsadanian, Maia ; Kim, Jungsu ; Li, Aimin ; Knoten, Amanda ; Jain, Sanjay ; Hirsch-Reinshagen, Veronica ; Wellington, Cheryl L. ; Bales, Kelly R. ; Paul, Steven M. ; Holtzman, David M. / Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease. In: Journal of Clinical Investigation. 2008 ; Vol. 118, No. 2. pp. 671-682.
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