Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle

M. P. Bracamonte, K. S. Rud, Whyte G. Owen, Virginia M Miller

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Experiments were designed to determine how ovariectomy modulates mitogenic factors in platelets and how these factors affect proliferation of coronary arterial smooth muscle. Platelet-derived growth factors (PDGFAB and PDGFBB), transforming growth factors (TGF-β1 and TGF-β2), and vascular endothelial growth factor (VEGF165) were quantified in platelet lysates and platelet-poor plasma from adult gonadally intact and ovariectomized female pigs by ELISA. Proliferation of cultured coronary arterial smooth muscle cells (SMCs) from both groups of pigs was determined in response to autologous or heterologous platelet lysates. Platelet concentrations of PDGFBB, but not PDGFAB, TGF-β1, and TGF-β2, increased with ovariectomy. VEGF165 was not detected in platelets from either group. Proliferation of SMCs from ovariectomized females was significantly greater on exposure to autologous or heterologous platelet lysates than proliferation of SMCs from intact females. These results indicate that ovariectomy increases concentrations of PDGFBB in platelets. Higher levels of PDGFBB in platelets in synergy with other platelet-derived products could contribute to increased proliferative arterial response to injury after ovariectomy.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume283
Issue number3 52-3
StatePublished - 2002

Fingerprint

Ovariectomy
Mitogens
Smooth Muscle
Blood Platelets
Smooth Muscle Myocytes
Swine
Platelet-Derived Growth Factor
Transforming Growth Factors
Vascular Endothelial Growth Factor A
Enzyme-Linked Immunosorbent Assay
Wounds and Injuries

Keywords

  • Estrogen
  • Hormone
  • Megakaryocytes
  • Platelet-derived growth factor
  • Transforming growth factor

ASJC Scopus subject areas

  • Physiology

Cite this

Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle. / Bracamonte, M. P.; Rud, K. S.; Owen, Whyte G.; Miller, Virginia M.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 283, No. 3 52-3, 2002.

Research output: Contribution to journalArticle

@article{53a75968b0dc440d84d3ba78d5ecc0d2,
title = "Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle",
abstract = "Experiments were designed to determine how ovariectomy modulates mitogenic factors in platelets and how these factors affect proliferation of coronary arterial smooth muscle. Platelet-derived growth factors (PDGFAB and PDGFBB), transforming growth factors (TGF-β1 and TGF-β2), and vascular endothelial growth factor (VEGF165) were quantified in platelet lysates and platelet-poor plasma from adult gonadally intact and ovariectomized female pigs by ELISA. Proliferation of cultured coronary arterial smooth muscle cells (SMCs) from both groups of pigs was determined in response to autologous or heterologous platelet lysates. Platelet concentrations of PDGFBB, but not PDGFAB, TGF-β1, and TGF-β2, increased with ovariectomy. VEGF165 was not detected in platelets from either group. Proliferation of SMCs from ovariectomized females was significantly greater on exposure to autologous or heterologous platelet lysates than proliferation of SMCs from intact females. These results indicate that ovariectomy increases concentrations of PDGFBB in platelets. Higher levels of PDGFBB in platelets in synergy with other platelet-derived products could contribute to increased proliferative arterial response to injury after ovariectomy.",
keywords = "Estrogen, Hormone, Megakaryocytes, Platelet-derived growth factor, Transforming growth factor",
author = "Bracamonte, {M. P.} and Rud, {K. S.} and Owen, {Whyte G.} and Miller, {Virginia M}",
year = "2002",
language = "English (US)",
volume = "283",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "3 52-3",

}

TY - JOUR

T1 - Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle

AU - Bracamonte, M. P.

AU - Rud, K. S.

AU - Owen, Whyte G.

AU - Miller, Virginia M

PY - 2002

Y1 - 2002

N2 - Experiments were designed to determine how ovariectomy modulates mitogenic factors in platelets and how these factors affect proliferation of coronary arterial smooth muscle. Platelet-derived growth factors (PDGFAB and PDGFBB), transforming growth factors (TGF-β1 and TGF-β2), and vascular endothelial growth factor (VEGF165) were quantified in platelet lysates and platelet-poor plasma from adult gonadally intact and ovariectomized female pigs by ELISA. Proliferation of cultured coronary arterial smooth muscle cells (SMCs) from both groups of pigs was determined in response to autologous or heterologous platelet lysates. Platelet concentrations of PDGFBB, but not PDGFAB, TGF-β1, and TGF-β2, increased with ovariectomy. VEGF165 was not detected in platelets from either group. Proliferation of SMCs from ovariectomized females was significantly greater on exposure to autologous or heterologous platelet lysates than proliferation of SMCs from intact females. These results indicate that ovariectomy increases concentrations of PDGFBB in platelets. Higher levels of PDGFBB in platelets in synergy with other platelet-derived products could contribute to increased proliferative arterial response to injury after ovariectomy.

AB - Experiments were designed to determine how ovariectomy modulates mitogenic factors in platelets and how these factors affect proliferation of coronary arterial smooth muscle. Platelet-derived growth factors (PDGFAB and PDGFBB), transforming growth factors (TGF-β1 and TGF-β2), and vascular endothelial growth factor (VEGF165) were quantified in platelet lysates and platelet-poor plasma from adult gonadally intact and ovariectomized female pigs by ELISA. Proliferation of cultured coronary arterial smooth muscle cells (SMCs) from both groups of pigs was determined in response to autologous or heterologous platelet lysates. Platelet concentrations of PDGFBB, but not PDGFAB, TGF-β1, and TGF-β2, increased with ovariectomy. VEGF165 was not detected in platelets from either group. Proliferation of SMCs from ovariectomized females was significantly greater on exposure to autologous or heterologous platelet lysates than proliferation of SMCs from intact females. These results indicate that ovariectomy increases concentrations of PDGFBB in platelets. Higher levels of PDGFBB in platelets in synergy with other platelet-derived products could contribute to increased proliferative arterial response to injury after ovariectomy.

KW - Estrogen

KW - Hormone

KW - Megakaryocytes

KW - Platelet-derived growth factor

KW - Transforming growth factor

UR - http://www.scopus.com/inward/record.url?scp=0036352540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036352540&partnerID=8YFLogxK

M3 - Article

C2 - 12181111

AN - SCOPUS:0036352540

VL - 283

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 3 52-3

ER -