Osteoprogenitor cells within skeletal muscle

P. Bosch, D. S. Musgrave, Yung Lee Joon Yung Lee, J. Cummins, F. Shuler, S. C. Ghivizzani, Christopher H Evans, P. D. Robbins, J. Huard

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

The formation of ectopic bone within skeletal muscle is a widely observed phenomenon. However, the source of the osteoprogenitor cells responsible for ectopic bone formation remains unknown. This study was designed to test for osteogenic differentiation among cells isolated from skeletal muscle tissue. Different subpopulations of cells derived from an adult mouse skeletal muscle were tested for induction of alkaline phosphatase activity after exposure to bone morphogenetic protein-2 in vitro. A responsive subpopulation was identified, transduced with a retrovirus encoding for β-galactosidase (Rv-lacZ) and an adenoviral construct encoding for one bone morphogenetic protein-2, and injected into the hindlimb of immune compromised (severe combined immunodeficient, or SCID) mice. The injected cells appeared to actively participate in the ectopic bone formation. The existence of lacZ-positive muscle-derived cells colocalized with osteocalcin-producing cells within lacunae of newly formed bone matrix suggests osteoblast and osteocyte differentiation. Although a specific cell was not isolated, these data support the contentions that osteoprogenitor cells reside within skeletal muscle and that muscle may represent a source other than bone marrow for the harvest of these cells.

Original languageEnglish (US)
Pages (from-to)933-944
Number of pages12
JournalJournal of Orthopaedic Research
Volume18
Issue number6
DOIs
StatePublished - Nov 2000
Externally publishedYes

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Skeletal Muscle
Osteogenesis
Bone Morphogenetic Protein 2
Galactosidases
Osteocytes
Muscles
Bone Matrix
SCID Mice
Osteocalcin
Retroviridae
Hindlimb
Osteoblasts
Bone Marrow Cells
Muscle Cells
Alkaline Phosphatase
Cell Differentiation

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Bosch, P., Musgrave, D. S., Joon Yung Lee, Y. L., Cummins, J., Shuler, F., Ghivizzani, S. C., ... Huard, J. (2000). Osteoprogenitor cells within skeletal muscle. Journal of Orthopaedic Research, 18(6), 933-944. https://doi.org/10.1002/jor.1100180613

Osteoprogenitor cells within skeletal muscle. / Bosch, P.; Musgrave, D. S.; Joon Yung Lee, Yung Lee; Cummins, J.; Shuler, F.; Ghivizzani, S. C.; Evans, Christopher H; Robbins, P. D.; Huard, J.

In: Journal of Orthopaedic Research, Vol. 18, No. 6, 11.2000, p. 933-944.

Research output: Contribution to journalArticle

Bosch, P, Musgrave, DS, Joon Yung Lee, YL, Cummins, J, Shuler, F, Ghivizzani, SC, Evans, CH, Robbins, PD & Huard, J 2000, 'Osteoprogenitor cells within skeletal muscle', Journal of Orthopaedic Research, vol. 18, no. 6, pp. 933-944. https://doi.org/10.1002/jor.1100180613
Bosch P, Musgrave DS, Joon Yung Lee YL, Cummins J, Shuler F, Ghivizzani SC et al. Osteoprogenitor cells within skeletal muscle. Journal of Orthopaedic Research. 2000 Nov;18(6):933-944. https://doi.org/10.1002/jor.1100180613
Bosch, P. ; Musgrave, D. S. ; Joon Yung Lee, Yung Lee ; Cummins, J. ; Shuler, F. ; Ghivizzani, S. C. ; Evans, Christopher H ; Robbins, P. D. ; Huard, J. / Osteoprogenitor cells within skeletal muscle. In: Journal of Orthopaedic Research. 2000 ; Vol. 18, No. 6. pp. 933-944.
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