Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials: Comparison of adenovirus mediated gene transfer and protein delivery of BMP-2

Lorenz Meinel, Sandra Hofmann, Oliver Betz, Robert Fajardo, Hans P. Merkle, Robert Langer, Christopher H Evans, Gordana Vunjak-Novakovic, David L. Kaplan

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Bone tissue engineering, gene therapy based on human mesenchymal stem cells (MSCs) and silk fibroin biomaterials were combined to study the impact of viral transfection on MSC osteogenic performance in vitro. MSCs were transduced with adenovirus containing a human BMP-2 (Ad-BMP-2) gene at clinically reasonable viral concentrations and cultured for 4 weeks. Controls with nontransfected MSCs, but exposed to exogenous BMP-2 concentrations on an analogous time profile as that secreted by the Ad-BMP-2 group, were compared. Both the Ad-BMP-2 MSC group and the exogenous protein BMP-2 group strongly expressed osteopontin and bone sialoprotein. Cells secreted a matrix that underwent mineralization on the silk fibroin scaffolds, forming clusters of osseous material, as determined by micro-computed tomography. The expression of osteogenic marker proteins and alkaline phosphatase was significantly higher in the Ad-BMP-2 MSC group than in the exogenous protein BMP-2 group, and no significant differences in mineralization were observed in two of the three MSC sources tested. The results demonstrate that transfection resulted in higher levels of expression of osteogenic marker genes, no change in proliferation rate and did not impact the capacity of the cells to calcify tissues on these protein scaffolds. These findings suggest additional options to control differentiation where exogenous additions of growth factors or morphogens can be replaced with transfected MSCs.

Original languageEnglish (US)
Pages (from-to)4993-5002
Number of pages10
JournalBiomaterials
Volume27
Issue number28
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Gene transfer
Silk
Biocompatible Materials
Stem cells
Mesenchymal Stromal Cells
Adenoviridae
Osteogenesis
Biomaterials
Proteins
Fibroins
Scaffolds
Transfection
Bone
Genes
Integrin-Binding Sialoprotein
Gene therapy
Osteopontin
Phosphoprotein Phosphatases
Phosphatases
Tissue Engineering

Keywords

  • Adenoviral gene transfer
  • bone morphogenetic protein (BMP-2)
  • Mesenchymal stem cells
  • Micro-computed tomography
  • Silk
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials : Comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. / Meinel, Lorenz; Hofmann, Sandra; Betz, Oliver; Fajardo, Robert; Merkle, Hans P.; Langer, Robert; Evans, Christopher H; Vunjak-Novakovic, Gordana; Kaplan, David L.

In: Biomaterials, Vol. 27, No. 28, 10.2006, p. 4993-5002.

Research output: Contribution to journalArticle

Meinel, Lorenz ; Hofmann, Sandra ; Betz, Oliver ; Fajardo, Robert ; Merkle, Hans P. ; Langer, Robert ; Evans, Christopher H ; Vunjak-Novakovic, Gordana ; Kaplan, David L. / Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials : Comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. In: Biomaterials. 2006 ; Vol. 27, No. 28. pp. 4993-5002.
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