Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components

Joseph P. Bidwell, Andre J van Wijnen, Edward G. Fey, Steven Dworetzky, Sheldon Penman, Janet L. Stein, Jane B. Lian, Gary S. Stein

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

The nuclear matrix appears to play an important role in developmental gene expression during osteoblast differentiation. To better understand this role, we examined nuclear matrix DNA-binding proteins that are sequence-specific and interact with the osteocalcin gene promoter. Multiple protein-DNA interactions involving two distinct nuclear matrix proteins occur within the 5′ regulatory sequences (nt -640 to -430). One of these proteins, NMP-1, is a ubiquitous, cell growth-regulated protein that is related to the transcription factor ATF and resides in both the nuclear matrix and the nonmatrix nuclear compartment. The other protein, NMP-2, is a cell type-specific, 38-kDa promoter factor that recognizes binding sites resembling the consensus site for the CCAAT/enhancer-binding protein C/EBP and is localized exclusively on the nuclear matrix. NMP-1 and NMP-2 each interact with two nuclear matrix protein-binding elements. These elements are present near key regulatory sites of the osteocalcin gene promoter, such as the principal steroid hormone (vitamin D)-responsive sequences. Binding in this region of the osteocalcin gene promoter suggests transient associations with the nuclear matrix that are distinct from the stable interactions of matrix attachment regions. Our results are consistent with involvement of the nuclear matrix in concentrating and/or localizing transcription factors that mediate the basal and steroid hormone responsiveness of osteocalcin gene transcription.

Original languageEnglish (US)
Pages (from-to)3162-3166
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number8
StatePublished - Apr 15 1993
Externally publishedYes

Fingerprint

Nuclear Matrix
Osteocalcin
Thromboplastin
Nuclear Matrix-Associated Proteins
Genes
Proteins
Matrix Attachment Regions
Steroids
Activating Transcription Factors
Hormones
CCAAT-Enhancer-Binding Proteins
Developmental Genes
DNA-Binding Proteins
Protein C
Osteoblasts
Vitamin D
Transcription Factors
Binding Sites
Gene Expression
DNA

Keywords

  • Chromatin structure
  • DNA-binding proteins
  • Gene expression
  • Osteoblast
  • Transcription

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Bidwell, J. P., van Wijnen, A. J., Fey, E. G., Dworetzky, S., Penman, S., Stein, J. L., ... Stein, G. S. (1993). Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components. Proceedings of the National Academy of Sciences of the United States of America, 90(8), 3162-3166.

Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components. / Bidwell, Joseph P.; van Wijnen, Andre J; Fey, Edward G.; Dworetzky, Steven; Penman, Sheldon; Stein, Janet L.; Lian, Jane B.; Stein, Gary S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 8, 15.04.1993, p. 3162-3166.

Research output: Contribution to journalArticle

Bidwell, JP, van Wijnen, AJ, Fey, EG, Dworetzky, S, Penman, S, Stein, JL, Lian, JB & Stein, GS 1993, 'Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components', Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 8, pp. 3162-3166.
Bidwell, Joseph P. ; van Wijnen, Andre J ; Fey, Edward G. ; Dworetzky, Steven ; Penman, Sheldon ; Stein, Janet L. ; Lian, Jane B. ; Stein, Gary S. / Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 8. pp. 3162-3166.
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