TY - JOUR
T1 - Osteoblasts secrete miRNA-containing extracellular vesicles that enhance expansion of human umbilical cord blood cells
AU - Morhayim, Jess
AU - Van De Peppel, Jeroen
AU - Braakman, Eric
AU - Rombouts, Elwin W.J.C.
AU - Ter Borg, Mariette N.D.
AU - Dudakovic, Amel
AU - Chiba, Hideki
AU - Van Der Eerden, Bram C.J.
AU - Raaijmakers, Marc H.
AU - Van Wijnen, Andre J.
AU - Cornelissen, Jan J.
AU - Van Leeuwen, Johannes P.
N1 - Publisher Copyright:
© The Author(s) 2016.
PY - 2016/9/2
Y1 - 2016/9/2
N2 - Osteolineage cells represent one of the critical bone marrow niche components that support maintenance of hematopoietic stem and progenitor cells (HSPCs). Recent studies demonstrate that extracellular vesicles (EVs) regulate stem cell development via horizontal transfer of bioactive cargo, including microRNAs (miRNAs). Using next-generation sequencing we show that human osteoblast-derived EVs contain highly abundant miRNAs specifically enriched in EVs, including critical regulators of hematopoietic proliferation (e.g., miR-29a). EV treatment of human umbilical cord blood-derived CD34 + HSPCs alters the expression of candidate miRNA targets, such as HBP1, BCL2 and PTEN. Furthermore, EVs enhance proliferation of CD34 + cells and their immature subsets in growth factor-driven ex vivo expansion cultures. Importantly, EV-expanded cells retain their differentiation capacity in vitro and successfully engraft in vivo. These discoveries reveal a novel osteoblast-derived EV-mediated mechanism for regulation of HSPC proliferation and warrant consideration of EV-miRNAs for the development of expansion strategies to treat hematological disorders.
AB - Osteolineage cells represent one of the critical bone marrow niche components that support maintenance of hematopoietic stem and progenitor cells (HSPCs). Recent studies demonstrate that extracellular vesicles (EVs) regulate stem cell development via horizontal transfer of bioactive cargo, including microRNAs (miRNAs). Using next-generation sequencing we show that human osteoblast-derived EVs contain highly abundant miRNAs specifically enriched in EVs, including critical regulators of hematopoietic proliferation (e.g., miR-29a). EV treatment of human umbilical cord blood-derived CD34 + HSPCs alters the expression of candidate miRNA targets, such as HBP1, BCL2 and PTEN. Furthermore, EVs enhance proliferation of CD34 + cells and their immature subsets in growth factor-driven ex vivo expansion cultures. Importantly, EV-expanded cells retain their differentiation capacity in vitro and successfully engraft in vivo. These discoveries reveal a novel osteoblast-derived EV-mediated mechanism for regulation of HSPC proliferation and warrant consideration of EV-miRNAs for the development of expansion strategies to treat hematological disorders.
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U2 - 10.1038/srep32034
DO - 10.1038/srep32034
M3 - Article
C2 - 27585950
AN - SCOPUS:84985905448
SN - 2045-2322
VL - 6
JO - Scientific reports
JF - Scientific reports
M1 - 32034
ER -