Osteoblast-specific gene expression after transplantation of marrow cells: Implications for skeletal gene therapy

Zhen Hou, Que Nguyen, Baruch Frenkel, Susan K. Nilsson, Moira Milne, Andre J van Wijnen, Janet L. Stein, Peter Quesenberry, Jane B. Lian, Gary S. Stein

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Somatic gene therapies require targeted transfer of the therapeutic gene(s) into stem cells that proliferate and then differentiate and express the gene in a tissue-restricted manner. We have developed an approach for gene therapy using marrow cells that takes advantage of the osteoblast specificity of the osteocalcin promoter to confine expression of chimeric genes to bone. Adherent marrow cells, carrying a reporter gene [chloramphenicol acetyltransferase (CAT)] under the control of a 1.7-kilobase rat osteocalcin gene promoter, were expanded ex vivo. After transplantation by intravenous infusion, engrafted donor cells in recipient mice were detected by the presence of the transgene in a broad spectrum of tissues. However, expression of the transgene was restricted to osteoblasts and osteocytes, as established by biochemical analysis of CAT activity and immunohistochemical analysis of CAT expression at the single cell level. Our data indicate that donor cells achieved long-term engraftment in various tissues of the recipients and that the CAT gene under control of the osteocalcin promoter is expressed specifically in bone. Thus, transplantation of multipotential marrow cells containing the osteocalcin promoter-controlled transgene provides an efficacious approach to deliver therapeutic gene expression to osteoblasts for treatment of bone disorders or tumor metastasis to the skeleton.

Original languageEnglish (US)
Pages (from-to)7294-7299
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number13
DOIs
StatePublished - Jun 22 1999
Externally publishedYes

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Cell Transplantation
Osteoblasts
Genetic Therapy
Chloramphenicol O-Acetyltransferase
Bone Marrow
Osteocalcin
Gene Expression
Transgenes
Bone and Bones
Genes
Transplantation
Osteocytes
Reporter Genes
Intravenous Infusions
Skeleton
Stem Cells
Therapeutics
Neoplasm Metastasis
Neoplasms

Keywords

  • Engraftment
  • Mesenchymal stem cells
  • Osteocalcin
  • Osteoprogenitors

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Osteoblast-specific gene expression after transplantation of marrow cells : Implications for skeletal gene therapy. / Hou, Zhen; Nguyen, Que; Frenkel, Baruch; Nilsson, Susan K.; Milne, Moira; van Wijnen, Andre J; Stein, Janet L.; Quesenberry, Peter; Lian, Jane B.; Stein, Gary S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 13, 22.06.1999, p. 7294-7299.

Research output: Contribution to journalArticle

Hou, Z, Nguyen, Q, Frenkel, B, Nilsson, SK, Milne, M, van Wijnen, AJ, Stein, JL, Quesenberry, P, Lian, JB & Stein, GS 1999, 'Osteoblast-specific gene expression after transplantation of marrow cells: Implications for skeletal gene therapy', Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 13, pp. 7294-7299. https://doi.org/10.1073/pnas.96.13.7294
Hou, Zhen ; Nguyen, Que ; Frenkel, Baruch ; Nilsson, Susan K. ; Milne, Moira ; van Wijnen, Andre J ; Stein, Janet L. ; Quesenberry, Peter ; Lian, Jane B. ; Stein, Gary S. / Osteoblast-specific gene expression after transplantation of marrow cells : Implications for skeletal gene therapy. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 13. pp. 7294-7299.
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