TY - JOUR
T1 - Osteoblast migration on poly(α-hydroxy esters)
AU - Ishaug, Susan L.
AU - Payne, Richard G.
AU - Yaszemski, Michael J.
AU - Aufdemorte, Thomas B.
AU - Bizios, Rena
AU - Mikos, Antonios G.
PY - 1996/5/20
Y1 - 1996/5/20
N2 - We investigated the migration of rat calvaria osteoblast populations on poly(α-hydroxy ester) films for up to 14 days to determine effects of substrate composition and culture conditions on the migratory characteristics of osteoblasts. Initial osteoblast culture conditions included cell colonies formed by seeding a high (84,000 cells/cm2) or low (42,000 cells/cm2) density of isolated osteoblasts on the polymer films, and bone tissue cultures formed by plating bone chips directly on the substrates. High density osteoblast colonies cultured and allowed to migrate and proliferate radially on 85: 15 poly(DL-lactic-co-glycolic acid) (PLGA) films, 75: 25 PLGA films, and tissue culture polystyrene controls demonstrated that the copolymer ratio in the polymer films did not affect the rate of increase in substrate surface area (or culture area) covered by the growing cell colony. However, the rate of increase in culture area was dependent on the initial osteoblast seeding density. Initial cell colonies formed with a lower osteoblast seeding density on 75:25 PLGA resulted in a lower rate of increase in culture area, specifically 4.9 ± 0.3 mm2/day, versus 14.1 ± 0.7 mm2/day for colonies seeded with a higher density of cells on the same polymer films. The proliferation rate for osteoblasts in the high and low density seeded osteoblast colonies did not differ, whereas the proliferation rate for the osteoblasts arising from the bone chips was lower than either of these isolated cell colonies. Confocal and light microscopy revealed that the osteoblast migration occurred as a monolayer of individual osteoblasts and not a calcified tissue front. These results demonstrated that cell seeding conditions strongly affect the rates of osteoblast migration and proliferation on biodegradable poly(α-hydroxy esters).
AB - We investigated the migration of rat calvaria osteoblast populations on poly(α-hydroxy ester) films for up to 14 days to determine effects of substrate composition and culture conditions on the migratory characteristics of osteoblasts. Initial osteoblast culture conditions included cell colonies formed by seeding a high (84,000 cells/cm2) or low (42,000 cells/cm2) density of isolated osteoblasts on the polymer films, and bone tissue cultures formed by plating bone chips directly on the substrates. High density osteoblast colonies cultured and allowed to migrate and proliferate radially on 85: 15 poly(DL-lactic-co-glycolic acid) (PLGA) films, 75: 25 PLGA films, and tissue culture polystyrene controls demonstrated that the copolymer ratio in the polymer films did not affect the rate of increase in substrate surface area (or culture area) covered by the growing cell colony. However, the rate of increase in culture area was dependent on the initial osteoblast seeding density. Initial cell colonies formed with a lower osteoblast seeding density on 75:25 PLGA resulted in a lower rate of increase in culture area, specifically 4.9 ± 0.3 mm2/day, versus 14.1 ± 0.7 mm2/day for colonies seeded with a higher density of cells on the same polymer films. The proliferation rate for osteoblasts in the high and low density seeded osteoblast colonies did not differ, whereas the proliferation rate for the osteoblasts arising from the bone chips was lower than either of these isolated cell colonies. Confocal and light microscopy revealed that the osteoblast migration occurred as a monolayer of individual osteoblasts and not a calcified tissue front. These results demonstrated that cell seeding conditions strongly affect the rates of osteoblast migration and proliferation on biodegradable poly(α-hydroxy esters).
KW - PLGA
KW - biodegradable polymers
KW - migration
KW - osteoblast
KW - poly(DL-lactic-co-glycolic acid)
KW - poly(α-hydroxy esters)
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=15844418449&partnerID=8YFLogxK
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U2 - 10.1002/(SICI)1097-0290(19960520)50:4<443::AID-BIT12>3.0.CO;2-K
DO - 10.1002/(SICI)1097-0290(19960520)50:4<443::AID-BIT12>3.0.CO;2-K
M3 - Article
C2 - 18626994
AN - SCOPUS:15844418449
SN - 0006-3592
VL - 50
SP - 443
EP - 451
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 4
ER -