Osteoblast growth and bone-healing response to three-dimensional poly(ε-caprolactone fumarate) scaffolds

Jinku Kim, Aditi Sharma, Brett Runge, Heather Waters, Bruce Doll, Sean Mcbride, Pedro Alvarez, Mahrokh Dadsetan, Michael J. Yaszemski, Jeffrey O. Hollinger

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Poly(ε-caprolactone fumarate) (PCLF) scaffold formulations were assessed as a delivery system for recombinant human bone morphogenetic protein (rhBMP-2) for bone tissue engineering. The formulations included PCLF with combinations of poly(vinyl alcohol) (PVA) and hydroxyapatite (HA). The assessments included in vitro and in vivo assays. In vitro assays validated cell attachment using a pre-osteoblast cell line (MC3T3-E1). Additionally, in vitro release profiles of rhBMP-2 from PCLF scaffolds were determined up to 21 days. The data suggested that PCLF incorporated with PVA and HA accelerated rhBMP-2 release and that the released protein was bioactive. For the in vivo study, a critical-sized defect (CSD) model in rabbit calvaria was used to test PCLF scaffolds. At 6 weeks post-implantation, significantly more bone formation was measured in PCLF scaffolds containing rhBMP-2 than in scaffolds without rhBMP-2. In conclusion, we demonstrated that PCLF delivered biologically active rhBMP-2, promoted bone healing in a CSD and has potential as a bone tissue engineering scaffold.

Original languageEnglish (US)
Pages (from-to)404-413
Number of pages10
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume6
Issue number5
DOIs
StatePublished - May 2012

Keywords

  • Bone tissue engineering
  • Poly(ε-caprolactone fumarate)
  • Rabbit calvarial critical-sized defect
  • RhBMP-2
  • Three-dimensional scaffold

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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    Kim, J., Sharma, A., Runge, B., Waters, H., Doll, B., Mcbride, S., Alvarez, P., Dadsetan, M., Yaszemski, M. J., & Hollinger, J. O. (2012). Osteoblast growth and bone-healing response to three-dimensional poly(ε-caprolactone fumarate) scaffolds. Journal of Tissue Engineering and Regenerative Medicine, 6(5), 404-413. https://doi.org/10.1002/term.442