Osteoblast function on synthetic biodegradable polymers

S. L. Ishaug, Michael J Yaszemski, R. Bizios, A. G. Mikos

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

Rat osteoblasts were cultured on films of biodegradable poly(L- lactic acid) (PLLA), 75:25 poly(DL-lactic-co-glycolic acid) (PLGA), 50:50 PLGA, and poly(glycolic acid) (PGA) for up to 14 days. Osteoblasts attached equally well to all the polymer substrates after 8 h in culture. By day 4 in culture, osteoblasts had exceeded confluency numbers, and their proliferation leveled off by day 7. An increase in alkaline phosphatase (ALP) activity from 1.92 (±0.47) x 10-7 for day 7 to 5.75 (±0.12) x 10-7 μmol/cell per min for day 14 was reported for osteoblasts cultured on 75:25 PLGA, which was comparable to that observed for tissue culture polystyrene (TCPS) controls. The ALP activities expressed by osteoblasts cultured on PLLA, 50:50 PLGA, and PGA films did not significantly increase over time. Collagen synthesis for osteoblasts cultured on all polymer substrates was similar to that of TCPS and did not vary with time. The morphology of cultured osteoblasts was not affected by the continuous degradation of the polymer substrates. These results demonstrate that poly(α-hydroxy esters) can provide a suitable substrate for osteoblast culture and hold promise in bone regeneration by osteoblast transplantation.

Original languageEnglish (US)
Pages (from-to)1445-1453
Number of pages9
JournalJournal of Biomedical Materials Research
Volume28
Issue number12
DOIs
StatePublished - Dec 1994
Externally publishedYes

Fingerprint

Biodegradable polymers
Osteoblasts
glycolic acid
Cell culture
Acids
Prostaglandins A
Tissue culture
Polymers
Polystyrenes
Phosphatases
Substrates
Lactic acid
Alkaline Phosphatase
Collagen
Rats
Esters
Bone
Degradation
Milk

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Osteoblast function on synthetic biodegradable polymers. / Ishaug, S. L.; Yaszemski, Michael J; Bizios, R.; Mikos, A. G.

In: Journal of Biomedical Materials Research, Vol. 28, No. 12, 12.1994, p. 1445-1453.

Research output: Contribution to journalArticle

Ishaug, S. L. ; Yaszemski, Michael J ; Bizios, R. ; Mikos, A. G. / Osteoblast function on synthetic biodegradable polymers. In: Journal of Biomedical Materials Research. 1994 ; Vol. 28, No. 12. pp. 1445-1453.
@article{2ee92462e45a41d6927ed2b8beb9dd2e,
title = "Osteoblast function on synthetic biodegradable polymers",
abstract = "Rat osteoblasts were cultured on films of biodegradable poly(L- lactic acid) (PLLA), 75:25 poly(DL-lactic-co-glycolic acid) (PLGA), 50:50 PLGA, and poly(glycolic acid) (PGA) for up to 14 days. Osteoblasts attached equally well to all the polymer substrates after 8 h in culture. By day 4 in culture, osteoblasts had exceeded confluency numbers, and their proliferation leveled off by day 7. An increase in alkaline phosphatase (ALP) activity from 1.92 (±0.47) x 10-7 for day 7 to 5.75 (±0.12) x 10-7 μmol/cell per min for day 14 was reported for osteoblasts cultured on 75:25 PLGA, which was comparable to that observed for tissue culture polystyrene (TCPS) controls. The ALP activities expressed by osteoblasts cultured on PLLA, 50:50 PLGA, and PGA films did not significantly increase over time. Collagen synthesis for osteoblasts cultured on all polymer substrates was similar to that of TCPS and did not vary with time. The morphology of cultured osteoblasts was not affected by the continuous degradation of the polymer substrates. These results demonstrate that poly(α-hydroxy esters) can provide a suitable substrate for osteoblast culture and hold promise in bone regeneration by osteoblast transplantation.",
author = "Ishaug, {S. L.} and Yaszemski, {Michael J} and R. Bizios and Mikos, {A. G.}",
year = "1994",
month = "12",
doi = "10.1002/jbm.820281210",
language = "English (US)",
volume = "28",
pages = "1445--1453",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "12",

}

TY - JOUR

T1 - Osteoblast function on synthetic biodegradable polymers

AU - Ishaug, S. L.

AU - Yaszemski, Michael J

AU - Bizios, R.

AU - Mikos, A. G.

PY - 1994/12

Y1 - 1994/12

N2 - Rat osteoblasts were cultured on films of biodegradable poly(L- lactic acid) (PLLA), 75:25 poly(DL-lactic-co-glycolic acid) (PLGA), 50:50 PLGA, and poly(glycolic acid) (PGA) for up to 14 days. Osteoblasts attached equally well to all the polymer substrates after 8 h in culture. By day 4 in culture, osteoblasts had exceeded confluency numbers, and their proliferation leveled off by day 7. An increase in alkaline phosphatase (ALP) activity from 1.92 (±0.47) x 10-7 for day 7 to 5.75 (±0.12) x 10-7 μmol/cell per min for day 14 was reported for osteoblasts cultured on 75:25 PLGA, which was comparable to that observed for tissue culture polystyrene (TCPS) controls. The ALP activities expressed by osteoblasts cultured on PLLA, 50:50 PLGA, and PGA films did not significantly increase over time. Collagen synthesis for osteoblasts cultured on all polymer substrates was similar to that of TCPS and did not vary with time. The morphology of cultured osteoblasts was not affected by the continuous degradation of the polymer substrates. These results demonstrate that poly(α-hydroxy esters) can provide a suitable substrate for osteoblast culture and hold promise in bone regeneration by osteoblast transplantation.

AB - Rat osteoblasts were cultured on films of biodegradable poly(L- lactic acid) (PLLA), 75:25 poly(DL-lactic-co-glycolic acid) (PLGA), 50:50 PLGA, and poly(glycolic acid) (PGA) for up to 14 days. Osteoblasts attached equally well to all the polymer substrates after 8 h in culture. By day 4 in culture, osteoblasts had exceeded confluency numbers, and their proliferation leveled off by day 7. An increase in alkaline phosphatase (ALP) activity from 1.92 (±0.47) x 10-7 for day 7 to 5.75 (±0.12) x 10-7 μmol/cell per min for day 14 was reported for osteoblasts cultured on 75:25 PLGA, which was comparable to that observed for tissue culture polystyrene (TCPS) controls. The ALP activities expressed by osteoblasts cultured on PLLA, 50:50 PLGA, and PGA films did not significantly increase over time. Collagen synthesis for osteoblasts cultured on all polymer substrates was similar to that of TCPS and did not vary with time. The morphology of cultured osteoblasts was not affected by the continuous degradation of the polymer substrates. These results demonstrate that poly(α-hydroxy esters) can provide a suitable substrate for osteoblast culture and hold promise in bone regeneration by osteoblast transplantation.

UR - http://www.scopus.com/inward/record.url?scp=0028657422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028657422&partnerID=8YFLogxK

U2 - 10.1002/jbm.820281210

DO - 10.1002/jbm.820281210

M3 - Article

C2 - 7876284

AN - SCOPUS:0028657422

VL - 28

SP - 1445

EP - 1453

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 12

ER -