Origins of circulating endothelial cells and endothelial outgrowth from blood

Yi Lin, Daniel J. Weisdorf, Anna Solovey, Robert P. Hebbel

Research output: Contribution to journalArticle

1194 Citations (Scopus)

Abstract

Normal adults have a small number of circulating endothelial cells (CEC) in peripheral blood, and endothelial outgrowth has been observed from cultures of blood. In this study we seek insight into the origins of CEC and endothelial outgrowth from cultures of blood. Fluorescence in situ hybridization analysis of blood samples from bone marrow transplant recipients who had received gender-mismatched transplants 5-20 months earlier showed that most CEC in fresh blood had recipient genotype. Endothelial outgrowth from the same blood samples after 9 days in culture (5-fold expansion) was still predominantly of the recipient genotype. In contrast, endothelial outgrowth after ~1 month (102-fold expansion) was mostly of donor genotype. Thus, recipient-genotype endothelial cells expanded only ~20-fold over this period, whereas donor-genotype endothelial cells expanded ~1000-fold. These data suggest that most CEC in fresh blood originate from vessel walls and have limited growth capability. Conversely, the data indicate that outgrowth of endothelial cells from cultures of blood is mostly derived from transplantable marrow-derived cells. Because these cells have more delayed outgrowth but a greater proliferative rate, our data suggest that they are derived from circulating angioblasts.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalJournal of Clinical Investigation
Volume105
Issue number1
StatePublished - Jan 2000
Externally publishedYes

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Endothelial Cells
Genotype
Bone Marrow
Tissue Donors
Fluorescence In Situ Hybridization
Blood Vessels
Cell Culture Techniques
Transplants
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Origins of circulating endothelial cells and endothelial outgrowth from blood. / Lin, Yi; Weisdorf, Daniel J.; Solovey, Anna; Hebbel, Robert P.

In: Journal of Clinical Investigation, Vol. 105, No. 1, 01.2000, p. 71-77.

Research output: Contribution to journalArticle

Lin, Y, Weisdorf, DJ, Solovey, A & Hebbel, RP 2000, 'Origins of circulating endothelial cells and endothelial outgrowth from blood', Journal of Clinical Investigation, vol. 105, no. 1, pp. 71-77.
Lin, Yi ; Weisdorf, Daniel J. ; Solovey, Anna ; Hebbel, Robert P. / Origins of circulating endothelial cells and endothelial outgrowth from blood. In: Journal of Clinical Investigation. 2000 ; Vol. 105, No. 1. pp. 71-77.
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