Organization of transcriptional regulatory machinery in osteoclast nuclei: Compartmentalization of Runx1

Laura H. Saltman, Amjad Javed, John Ribadeneyra, Sadiq Hussain, Daniel W. Young, Philip Osdoby, Alla Amcheslavsky, Andre J. Van Wijnen, Janet L. Stein, Gary S. Stein, Jane B. Lian, Zvi Bar-Shavit

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The osteoclast is a highly polarized multinucleated cell that resorbs bone. Using high resolution immunofluorescence microscopy, we demonstrated that all nuclei of an osteoclast are transcriptionally active. Each nucleus within the osteoclast contains punctately organized microenvironments where regulatory complexes that support transcriptional and post-transcriptional control reside. Functional equivalency of osteoclast nuclei is reflected by similar representation of regulatory proteins that support ribosomal RNA synthesis (nucleolin), mRNA transcription (RNA polymerase II, bromouridine triphosphate), processing of gene transcripts (SC3S), signal transduction (NF-κB), and phenotypic gene expression (Runx1). Our results establish that gene regulatory machinery is architecturally associated and compartmentalized within intranuclear microenvironments of the multiple nuclei of osteoclasts to support physiologically responsive modifications in cellular structural and functional properties.

Original languageEnglish (US)
Pages (from-to)871-880
Number of pages10
JournalJournal of Cellular Physiology
Issue number3
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


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