Organic osmolyte distribution and levels in the mammalian urinary bladder in diuresis and antidiuresis

Eugene D Kwon, John A. Dooley, Kyu Yong Jung, Peter M. Andrews, Arlyn García-Pérez, Maurice B. Burg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Inositol, sorbitol, glycerophosphocholine (GPC), and betaine are organic osmolytes that are accumulated by renal medullary cells in response to hyperosmotic stress. Previous screening studies, using nuclear magnetic resonance spectroscopy, have shown some of these same compounds to be present in extracts of whole urinary bladder from rabbits and rats. In the present study, we used highperformance liquid chromatography to quantify levels of these compounds in the separated epithelium and muscle of bladders taken from normal rabbits as well as diuretic and thirsted rats. We find that 1) high concentrations of organic osmolytes, namely inositol, GPC, and sorbitol, are present in urinary bladder; 2) levels of these osmolytes in the bladder epithelium are higher than in the muscle; 3) increased urinary osmolality due to antidiuresis is associated with a 2.4-fold increase in total osmolyte levels in rat bladder epithelium and a lesser (1.5-fold) increase in the muscle, compared with corresponding levels in tissues from diuretic animals; and 4) these increases in total osmolyte amounts in the epithelium are due to increased levels of GPC, sorbitol, and perhaps inositol (P = 0.07), whereas only GPC increases in the bladder muscle.

Original languageEnglish (US)
JournalAmerican Journal of Physiology
Volume271
Issue number1 PART 2
StatePublished - 1996
Externally publishedYes

Fingerprint

Diuresis
Urinary Bladder
Sorbitol
Inositol
Epithelium
Muscles
Diuretics
Rabbits
Betaine
Liquid Chromatography
Osmolar Concentration
Magnetic Resonance Spectroscopy
Kidney

Keywords

  • Bladder epithelium
  • Bladder muscle
  • Glycerophosphocholine
  • Inositol; betaine
  • Sorbitol
  • Urea

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Kwon, E. D., Dooley, J. A., Jung, K. Y., Andrews, P. M., García-Pérez, A., & Burg, M. B. (1996). Organic osmolyte distribution and levels in the mammalian urinary bladder in diuresis and antidiuresis. American Journal of Physiology, 271(1 PART 2).

Organic osmolyte distribution and levels in the mammalian urinary bladder in diuresis and antidiuresis. / Kwon, Eugene D; Dooley, John A.; Jung, Kyu Yong; Andrews, Peter M.; García-Pérez, Arlyn; Burg, Maurice B.

In: American Journal of Physiology, Vol. 271, No. 1 PART 2, 1996.

Research output: Contribution to journalArticle

Kwon, ED, Dooley, JA, Jung, KY, Andrews, PM, García-Pérez, A & Burg, MB 1996, 'Organic osmolyte distribution and levels in the mammalian urinary bladder in diuresis and antidiuresis', American Journal of Physiology, vol. 271, no. 1 PART 2.
Kwon, Eugene D ; Dooley, John A. ; Jung, Kyu Yong ; Andrews, Peter M. ; García-Pérez, Arlyn ; Burg, Maurice B. / Organic osmolyte distribution and levels in the mammalian urinary bladder in diuresis and antidiuresis. In: American Journal of Physiology. 1996 ; Vol. 271, No. 1 PART 2.
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