Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution

Matthew S. Kokotilo, Jodi Carter, Aducio Thiesen, Ming H. Chen, Angela Ochs, Rachel G. Khadaroo, Thomas A. Churchill

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Introduction: Our lab has developed an effective nutrient-rich solution that facilitates energy production and control of oxidative stress during static cold storage of the intestine; however, the requirement for oncotic agents, such as hydroxyethylstarch (HES), has not been evaluated. This study investigated the effectiveness and requirement for HES in an intraluminal preservation solution during a clinically relevant period of cold storage. Methods: Rat intestines were procured, including an intravascular flush with University of Wisconsin solution followed by a 'back table' intraluminal flush with a nutrient-rich preservation solution containing varying amounts of HES ( n= 6 per group): Group 1, 0%; Group 2, 2.5%; Group 3, 5%; Group 4, 10%. Energetics, oxidative stress, and morphology were assessed over a 24. h time-course of cold storage. Results: Overall, the 5% HES solution, Group 3, demonstrated superior energetic status (ATP and total adenylates) compared to all groups,. P< 0.05. Malondialdehyde levels indicated a reduction in oxidative stress in Groups 3 and 4 ( P< 0.05). After 12. h, median modified Parks' grades for Groups 2 and 3 were significantly lower than Groups 1 and 4,. P< 0.05. Conclusion: Our data suggests that when employing an intraluminal preservation solution for static organ storage, oncotic support is a fundamental requirement; 5% HES is optimal.

Original languageEnglish (US)
Pages (from-to)236-242
Number of pages7
JournalCryobiology
Volume61
Issue number3
DOIs
StatePublished - Dec 1 2010

Keywords

  • Energetic stress
  • Intestinal-specific
  • Intraluminal preservation solution
  • Oncotic/osmotic agents
  • Organ preservation
  • Osmotic stress
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Kokotilo, M. S., Carter, J., Thiesen, A., Chen, M. H., Ochs, A., Khadaroo, R. G., & Churchill, T. A. (2010). Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution. Cryobiology, 61(3), 236-242. https://doi.org/10.1016/j.cryobiol.2010.08.001