Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution

Matthew S. Kokotilo, Jodi Carter, Aducio Thiesen, Ming H. Chen, Angela Ochs, Rachel G. Khadaroo, Thomas A. Churchill

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction: Our lab has developed an effective nutrient-rich solution that facilitates energy production and control of oxidative stress during static cold storage of the intestine; however, the requirement for oncotic agents, such as hydroxyethylstarch (HES), has not been evaluated. This study investigated the effectiveness and requirement for HES in an intraluminal preservation solution during a clinically relevant period of cold storage. Methods: Rat intestines were procured, including an intravascular flush with University of Wisconsin solution followed by a 'back table' intraluminal flush with a nutrient-rich preservation solution containing varying amounts of HES ( n= 6 per group): Group 1, 0%; Group 2, 2.5%; Group 3, 5%; Group 4, 10%. Energetics, oxidative stress, and morphology were assessed over a 24. h time-course of cold storage. Results: Overall, the 5% HES solution, Group 3, demonstrated superior energetic status (ATP and total adenylates) compared to all groups,. P< 0.05. Malondialdehyde levels indicated a reduction in oxidative stress in Groups 3 and 4 ( P< 0.05). After 12. h, median modified Parks' grades for Groups 2 and 3 were significantly lower than Groups 1 and 4,. P< 0.05. Conclusion: Our data suggests that when employing an intraluminal preservation solution for static organ storage, oncotic support is a fundamental requirement; 5% HES is optimal.

Original languageEnglish (US)
Pages (from-to)236-242
Number of pages7
JournalCryobiology
Volume61
Issue number3
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

Fingerprint

Cold storage
Oxidative stress
Oxidative Stress
cold storage
oxidative stress
Intestines
Organ Preservation Solutions
Nutrients
intestines
Food
storage organs
Malondialdehyde
nutrients
malondialdehyde
Adenosine Triphosphate
Rats
rats
energy
methodology

Keywords

  • Energetic stress
  • Intestinal-specific
  • Intraluminal preservation solution
  • Oncotic/osmotic agents
  • Organ preservation
  • Osmotic stress
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kokotilo, M. S., Carter, J., Thiesen, A., Chen, M. H., Ochs, A., Khadaroo, R. G., & Churchill, T. A. (2010). Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution. Cryobiology, 61(3), 236-242. https://doi.org/10.1016/j.cryobiol.2010.08.001

Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution. / Kokotilo, Matthew S.; Carter, Jodi; Thiesen, Aducio; Chen, Ming H.; Ochs, Angela; Khadaroo, Rachel G.; Churchill, Thomas A.

In: Cryobiology, Vol. 61, No. 3, 01.12.2010, p. 236-242.

Research output: Contribution to journalArticle

Kokotilo, MS, Carter, J, Thiesen, A, Chen, MH, Ochs, A, Khadaroo, RG & Churchill, TA 2010, 'Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution', Cryobiology, vol. 61, no. 3, pp. 236-242. https://doi.org/10.1016/j.cryobiol.2010.08.001
Kokotilo, Matthew S. ; Carter, Jodi ; Thiesen, Aducio ; Chen, Ming H. ; Ochs, Angela ; Khadaroo, Rachel G. ; Churchill, Thomas A. / Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution. In: Cryobiology. 2010 ; Vol. 61, No. 3. pp. 236-242.
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