Optimization of polycaprolactone fibrous scaffold for heart valve tissue engineering

Soumen Jana, Amrita Bhagia, Amir Lerman

Research output: Contribution to journalArticle

Abstract

Pore size is generally small in nanofibrous scaffolds prepared by electrospinning polymeric solutions. Increase of scaffold thickness leads to decrease in pore size, causing impediment to cell infiltration into the scaffolds during tissue engineering. In contrast, comparatively larger pore size can be realized in microfibrous scaffolds prepared from polymeric solutions at higher concentrations. Further, microfibrous scaffolds are conducive to infiltration of reparative M2 phenotype macrophages during in vivo/in situ tissue engineering. However, rise of mechanical properties of a fibrous scaffold with the increase of polymer concentration may limit the functionality of a scaffold-based, tissue-engineered heart valve. In this study, we developed microfibrous scaffolds from 14%, 16% and 18% (wt/v) polycaprolactone (PCL) polymer solutions prepared with chloroform solvent. Porcine valvular interstitial cells were cultured in the scaffolds for 14 d to investigate the effect of microfibers prepared with different PCL concentrations on the seeded cells. Further, fresh microfibrous scaffolds were implanted subcutaneously in a rat model for two months to investigate the effect of microfibers on infiltrated cells. Cell proliferation, and its morphologies, gene expression and deposition of different extracellular matrix proteins in the in vitro study were characterized. During the in vivo study, we characterized cell infiltration, and myofibroblast and M1/M2 phenotypes expression of the infiltrated cells. Among different PCL concentrations, microfibrous scaffolds from 14% solution were suitable for heart valve tissue engineering for their sufficient pore size and low but adequate tensile properties, which promoted cell adhesion to and proliferation in the scaffolds, and effective gene expression and extracellular matrix deposition by the cells in vitro. They also encouraged the cells in vivo for their infiltration and effective gene expression, including M2 phenotype expression.

Original languageEnglish (US)
Number of pages1
JournalBiomedical materials (Bristol, England)
Volume14
Issue number6
DOIs
StatePublished - Oct 8 2019

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Polycaprolactone
Scaffolds (biology)
Tissue engineering
Scaffolds
Infiltration
Pore size
Gene expression
polycaprolactone
Macrophages
Extracellular Matrix Proteins
Cell adhesion
Cell proliferation
Electrospinning
Polymer solutions
Chloroform
Chlorine compounds
Tensile properties
Rats
Polymers
Tissue

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Optimization of polycaprolactone fibrous scaffold for heart valve tissue engineering. / Jana, Soumen; Bhagia, Amrita; Lerman, Amir.

In: Biomedical materials (Bristol, England), Vol. 14, No. 6, 08.10.2019.

Research output: Contribution to journalArticle

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