Optimization of paired pulse voltammetry using sawhorse waveform

Yoonbae Oh, Do Hyoung Kim, Hojin Shin, Cheonho Park, Su-Youne Chang, Charles D. Blaha, Kevin E. Bennet, In Young Kim, Kendall H Lee, Dong Pyo Jang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The influence of the reduction process on paired-pulse voltammetry (PPV), which consists of two identical triangle-shaped pulses, was studied in this study by increasing peak potential duration. We propose sawhorse waveform as an optimized PPV waveform for enhancing the responses of PPV on dopamine and serotonin (5-HT). To investigate the influence of the scanning parameters on the reduction process and response of PPV, the responses of dopamine were measured by changing the switching potential (from 1.0V to 1.5V), and scan rates (from 400V/s to 1000V/s) based on triangle shaped PPV. As a result, the oxidation and reduction peak ratio in the response of the primary pulse in PPV decreased in a linear fashion with increases to the duration between oxidation and reduction potential, which lead us to obtain more defined features in the subtracted voltammograms (primary-secondary) in PPV. Based on these results, sawhorse waveform PPV with prolonged switching potential duration was suggested to increase the sensitivity of PPV while maintaining its own advantages, and it is also confirmed in an in vivo experiment. In addition to the dopamine experiments, we also conducted an experiment with serotonin utilizing the N-shaped sawhorse waveform PPV. The subtracted voltammogram in the N-shaped PPV with 1.0V peak and 4ms peak potential duration clearly showed the characteristics of serotonin. In conclusion, PPV waveforms could be optimized to improve the sensitivity of PPV responses by the control of the reduction process.

Original languageEnglish (US)
Pages (from-to)10061-10073
Number of pages13
JournalInternational Journal of Electrochemical Science
Volume10
Issue number12
StatePublished - 2015

Fingerprint

Voltammetry
Serotonin
Dopamine
Oxidation
Experiments

Keywords

  • Dopamine
  • Fast-scan cyclic voltammetry (FSCV)
  • Paired pulse voltammetry (PPV)
  • Sawhorse waveform
  • Serotonin

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Oh, Y., Kim, D. H., Shin, H., Park, C., Chang, S-Y., Blaha, C. D., ... Jang, D. P. (2015). Optimization of paired pulse voltammetry using sawhorse waveform. International Journal of Electrochemical Science, 10(12), 10061-10073.

Optimization of paired pulse voltammetry using sawhorse waveform. / Oh, Yoonbae; Kim, Do Hyoung; Shin, Hojin; Park, Cheonho; Chang, Su-Youne; Blaha, Charles D.; Bennet, Kevin E.; Kim, In Young; Lee, Kendall H; Jang, Dong Pyo.

In: International Journal of Electrochemical Science, Vol. 10, No. 12, 2015, p. 10061-10073.

Research output: Contribution to journalArticle

Oh, Y, Kim, DH, Shin, H, Park, C, Chang, S-Y, Blaha, CD, Bennet, KE, Kim, IY, Lee, KH & Jang, DP 2015, 'Optimization of paired pulse voltammetry using sawhorse waveform', International Journal of Electrochemical Science, vol. 10, no. 12, pp. 10061-10073.
Oh, Yoonbae ; Kim, Do Hyoung ; Shin, Hojin ; Park, Cheonho ; Chang, Su-Youne ; Blaha, Charles D. ; Bennet, Kevin E. ; Kim, In Young ; Lee, Kendall H ; Jang, Dong Pyo. / Optimization of paired pulse voltammetry using sawhorse waveform. In: International Journal of Electrochemical Science. 2015 ; Vol. 10, No. 12. pp. 10061-10073.
@article{ce704e0e34e6444dab5f2b6b520aa093,
title = "Optimization of paired pulse voltammetry using sawhorse waveform",
abstract = "The influence of the reduction process on paired-pulse voltammetry (PPV), which consists of two identical triangle-shaped pulses, was studied in this study by increasing peak potential duration. We propose sawhorse waveform as an optimized PPV waveform for enhancing the responses of PPV on dopamine and serotonin (5-HT). To investigate the influence of the scanning parameters on the reduction process and response of PPV, the responses of dopamine were measured by changing the switching potential (from 1.0V to 1.5V), and scan rates (from 400V/s to 1000V/s) based on triangle shaped PPV. As a result, the oxidation and reduction peak ratio in the response of the primary pulse in PPV decreased in a linear fashion with increases to the duration between oxidation and reduction potential, which lead us to obtain more defined features in the subtracted voltammograms (primary-secondary) in PPV. Based on these results, sawhorse waveform PPV with prolonged switching potential duration was suggested to increase the sensitivity of PPV while maintaining its own advantages, and it is also confirmed in an in vivo experiment. In addition to the dopamine experiments, we also conducted an experiment with serotonin utilizing the N-shaped sawhorse waveform PPV. The subtracted voltammogram in the N-shaped PPV with 1.0V peak and 4ms peak potential duration clearly showed the characteristics of serotonin. In conclusion, PPV waveforms could be optimized to improve the sensitivity of PPV responses by the control of the reduction process.",
keywords = "Dopamine, Fast-scan cyclic voltammetry (FSCV), Paired pulse voltammetry (PPV), Sawhorse waveform, Serotonin",
author = "Yoonbae Oh and Kim, {Do Hyoung} and Hojin Shin and Cheonho Park and Su-Youne Chang and Blaha, {Charles D.} and Bennet, {Kevin E.} and Kim, {In Young} and Lee, {Kendall H} and Jang, {Dong Pyo}",
year = "2015",
language = "English (US)",
volume = "10",
pages = "10061--10073",
journal = "International Journal of Electrochemical Science",
issn = "1452-3981",
publisher = "Electrochemical Science Group",
number = "12",

}

TY - JOUR

T1 - Optimization of paired pulse voltammetry using sawhorse waveform

AU - Oh, Yoonbae

AU - Kim, Do Hyoung

AU - Shin, Hojin

AU - Park, Cheonho

AU - Chang, Su-Youne

AU - Blaha, Charles D.

AU - Bennet, Kevin E.

AU - Kim, In Young

AU - Lee, Kendall H

AU - Jang, Dong Pyo

PY - 2015

Y1 - 2015

N2 - The influence of the reduction process on paired-pulse voltammetry (PPV), which consists of two identical triangle-shaped pulses, was studied in this study by increasing peak potential duration. We propose sawhorse waveform as an optimized PPV waveform for enhancing the responses of PPV on dopamine and serotonin (5-HT). To investigate the influence of the scanning parameters on the reduction process and response of PPV, the responses of dopamine were measured by changing the switching potential (from 1.0V to 1.5V), and scan rates (from 400V/s to 1000V/s) based on triangle shaped PPV. As a result, the oxidation and reduction peak ratio in the response of the primary pulse in PPV decreased in a linear fashion with increases to the duration between oxidation and reduction potential, which lead us to obtain more defined features in the subtracted voltammograms (primary-secondary) in PPV. Based on these results, sawhorse waveform PPV with prolonged switching potential duration was suggested to increase the sensitivity of PPV while maintaining its own advantages, and it is also confirmed in an in vivo experiment. In addition to the dopamine experiments, we also conducted an experiment with serotonin utilizing the N-shaped sawhorse waveform PPV. The subtracted voltammogram in the N-shaped PPV with 1.0V peak and 4ms peak potential duration clearly showed the characteristics of serotonin. In conclusion, PPV waveforms could be optimized to improve the sensitivity of PPV responses by the control of the reduction process.

AB - The influence of the reduction process on paired-pulse voltammetry (PPV), which consists of two identical triangle-shaped pulses, was studied in this study by increasing peak potential duration. We propose sawhorse waveform as an optimized PPV waveform for enhancing the responses of PPV on dopamine and serotonin (5-HT). To investigate the influence of the scanning parameters on the reduction process and response of PPV, the responses of dopamine were measured by changing the switching potential (from 1.0V to 1.5V), and scan rates (from 400V/s to 1000V/s) based on triangle shaped PPV. As a result, the oxidation and reduction peak ratio in the response of the primary pulse in PPV decreased in a linear fashion with increases to the duration between oxidation and reduction potential, which lead us to obtain more defined features in the subtracted voltammograms (primary-secondary) in PPV. Based on these results, sawhorse waveform PPV with prolonged switching potential duration was suggested to increase the sensitivity of PPV while maintaining its own advantages, and it is also confirmed in an in vivo experiment. In addition to the dopamine experiments, we also conducted an experiment with serotonin utilizing the N-shaped sawhorse waveform PPV. The subtracted voltammogram in the N-shaped PPV with 1.0V peak and 4ms peak potential duration clearly showed the characteristics of serotonin. In conclusion, PPV waveforms could be optimized to improve the sensitivity of PPV responses by the control of the reduction process.

KW - Dopamine

KW - Fast-scan cyclic voltammetry (FSCV)

KW - Paired pulse voltammetry (PPV)

KW - Sawhorse waveform

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=84953320430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953320430&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84953320430

VL - 10

SP - 10061

EP - 10073

JO - International Journal of Electrochemical Science

JF - International Journal of Electrochemical Science

SN - 1452-3981

IS - 12

ER -