Optimal fluorescein dose for intravenous application in miniprobe-based confocal laser scanning microscopy in pigs.

Valentin Becker, Frank J. van den Broek, Anna M. Buchner, Evelien Dekker, Michael B. Wallace, Stefan von Delius, Armin Schneider, Roland M. Schmid, Alexander Meining

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Probe-based confocal laser scanning endomicroscopy (pCLE) enables in-vivo histopathology during ongoing endoscopy. The most frequently used fluorophore is fluorescein sodium administered intravenously. Despite the increased use of pCLE, there are hardly any data on the ideal fluorescein concentration. Therefore, rectal mucosa of pigs was examined after injection (i.v.) of fluorescein as a single bolus (0.1 ml/kg body weight) in different concentrations (0.5%, 1%, 2%, 5%, 10%). Video sequences were recorded after 1, 5 and 60 min. For objective evaluation signal-to-noise ratio (SNR) was computed. For subjective evaluation, video sequences were randomized and blindly evaluated by experienced endomicroscopists. In total, 19037 images were analyzed. The mean SNR increased from the lowest (0.5%; SNR 6.75, range 3.55) to the highest concentration (10%; SNR 9.11, range 3.18). Subjective evaluation demonstrated best image quality with fluorescein concentration of 5%. In conclusion, pCLE shows best results using single injection of IV fluorescein 5%.

Original languageEnglish (US)
Pages (from-to)108-113
Number of pages6
JournalJournal of biophotonics
Volume4
Issue number1-2
DOIs
StatePublished - Jan 2011

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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    Becker, V., van den Broek, F. J., Buchner, A. M., Dekker, E., Wallace, M. B., von Delius, S., Schneider, A., Schmid, R. M., & Meining, A. (2011). Optimal fluorescein dose for intravenous application in miniprobe-based confocal laser scanning microscopy in pigs. Journal of biophotonics, 4(1-2), 108-113. https://doi.org/10.1002/jbio.201000028