TY - JOUR
T1 - Optimal fluorescein dose for intravenous application in miniprobe-based confocal laser scanning microscopy in pigs.
AU - Becker, Valentin
AU - van den Broek, Frank J.
AU - Buchner, Anna M.
AU - Dekker, Evelien
AU - Wallace, Michael B.
AU - von Delius, Stefan
AU - Schneider, Armin
AU - Schmid, Roland M.
AU - Meining, Alexander
PY - 2011/1
Y1 - 2011/1
N2 - Probe-based confocal laser scanning endomicroscopy (pCLE) enables in-vivo histopathology during ongoing endoscopy. The most frequently used fluorophore is fluorescein sodium administered intravenously. Despite the increased use of pCLE, there are hardly any data on the ideal fluorescein concentration. Therefore, rectal mucosa of pigs was examined after injection (i.v.) of fluorescein as a single bolus (0.1 ml/kg body weight) in different concentrations (0.5%, 1%, 2%, 5%, 10%). Video sequences were recorded after 1, 5 and 60 min. For objective evaluation signal-to-noise ratio (SNR) was computed. For subjective evaluation, video sequences were randomized and blindly evaluated by experienced endomicroscopists. In total, 19037 images were analyzed. The mean SNR increased from the lowest (0.5%; SNR 6.75, range 3.55) to the highest concentration (10%; SNR 9.11, range 3.18). Subjective evaluation demonstrated best image quality with fluorescein concentration of 5%. In conclusion, pCLE shows best results using single injection of IV fluorescein 5%.
AB - Probe-based confocal laser scanning endomicroscopy (pCLE) enables in-vivo histopathology during ongoing endoscopy. The most frequently used fluorophore is fluorescein sodium administered intravenously. Despite the increased use of pCLE, there are hardly any data on the ideal fluorescein concentration. Therefore, rectal mucosa of pigs was examined after injection (i.v.) of fluorescein as a single bolus (0.1 ml/kg body weight) in different concentrations (0.5%, 1%, 2%, 5%, 10%). Video sequences were recorded after 1, 5 and 60 min. For objective evaluation signal-to-noise ratio (SNR) was computed. For subjective evaluation, video sequences were randomized and blindly evaluated by experienced endomicroscopists. In total, 19037 images were analyzed. The mean SNR increased from the lowest (0.5%; SNR 6.75, range 3.55) to the highest concentration (10%; SNR 9.11, range 3.18). Subjective evaluation demonstrated best image quality with fluorescein concentration of 5%. In conclusion, pCLE shows best results using single injection of IV fluorescein 5%.
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U2 - 10.1002/jbio.201000028
DO - 10.1002/jbio.201000028
M3 - Article
C2 - 20533429
AN - SCOPUS:79953160705
SN - 1864-063X
VL - 4
SP - 108
EP - 113
JO - Journal of biophotonics
JF - Journal of biophotonics
IS - 1-2
ER -