Operating characteristics of the hypothalamo-pituitary-gonadal axis in men: Circadian, ultradian, and pulsatile release of prolactin and its temporal coupling with luteinizing hormone

Johannes D Veldhuis, M. L. Johnson

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Abstract

To invetigate the circadian, ultradian, and pulsatile nature of PRL release in the human, we sampled blood at 10-min intervals for 24 h in each of 12 normal young men. The subsequent serum PRL time series were subjected to contemporary techniques of rhythmic and episodic peak detection. Fourier analysis revealed a significant circadian rhythm in serum PRL concentrations in all 12 men. The mean circadian amplitude was 2.1 ± 0.3 ng/mL (μg/L), which accounted for an average of 30 ± 4% of the 24-h mean PRL concentration. In addition, multiple ultradian PRL rhythms were found with periodicities ranging from 22-242 min. Spectral analysis disclosed ultradian cycles with periodicities of 30-32, 51-59, 90-98, and 234 min. Assessment of episodic PRL pulsatility by Cluster analysis revealed 14 ± 1 PRL peaks/24 h (P < 0.01 vs. signal-free noise), which occurred at an interpulse interval of 95 ± 6 min. The average duration of a serum PRL peak was 67 ± 5 min, and its incremental amplitude was 4.0 ± 0.3 ng/ml (μg/L), which represented a 58 ± 6% increase above the preceding nadir. Discrete PRL peaks were separated by nonpulsatile valleys, with a mean duration of 27 ± 1 min. Analysis of the temporal coupling between LH and PRL release by bivariate autoregressive modeling in six men revealed significant cross-correlations between LH and simultaneous PRL concentrations as well as between LH and PRL concentrations that lagged LH by 10 or 20 min. Cross-spectral analysis demonstrated significantly correlated PRL and LH cycles with periodicities of 33-37, 47-52, and 84-106 min. In summary, PRL release in normal young men is characterized by significant circadian and ultradian periodicities, by discrete episodic pulsations that occur approximately every 95 min, and by a close temporal coupling with LH (temporal lag between LH and PRL of 0-20 min).

Original languageEnglish (US)
Pages (from-to)116-123
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume67
Issue number1
StatePublished - 1988
Externally publishedYes

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Periodicity
Luteinizing Hormone
Prolactin
Spectrum analysis
Fourier analysis
Cluster analysis
Time series
Blood
Serum
Fourier Analysis
Circadian Rhythm
Cluster Analysis
Noise
Ultradian Rhythm

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{a5850d3ea78f4e2f927119127e5727c9,
title = "Operating characteristics of the hypothalamo-pituitary-gonadal axis in men: Circadian, ultradian, and pulsatile release of prolactin and its temporal coupling with luteinizing hormone",
abstract = "To invetigate the circadian, ultradian, and pulsatile nature of PRL release in the human, we sampled blood at 10-min intervals for 24 h in each of 12 normal young men. The subsequent serum PRL time series were subjected to contemporary techniques of rhythmic and episodic peak detection. Fourier analysis revealed a significant circadian rhythm in serum PRL concentrations in all 12 men. The mean circadian amplitude was 2.1 ± 0.3 ng/mL (μg/L), which accounted for an average of 30 ± 4{\%} of the 24-h mean PRL concentration. In addition, multiple ultradian PRL rhythms were found with periodicities ranging from 22-242 min. Spectral analysis disclosed ultradian cycles with periodicities of 30-32, 51-59, 90-98, and 234 min. Assessment of episodic PRL pulsatility by Cluster analysis revealed 14 ± 1 PRL peaks/24 h (P < 0.01 vs. signal-free noise), which occurred at an interpulse interval of 95 ± 6 min. The average duration of a serum PRL peak was 67 ± 5 min, and its incremental amplitude was 4.0 ± 0.3 ng/ml (μg/L), which represented a 58 ± 6{\%} increase above the preceding nadir. Discrete PRL peaks were separated by nonpulsatile valleys, with a mean duration of 27 ± 1 min. Analysis of the temporal coupling between LH and PRL release by bivariate autoregressive modeling in six men revealed significant cross-correlations between LH and simultaneous PRL concentrations as well as between LH and PRL concentrations that lagged LH by 10 or 20 min. Cross-spectral analysis demonstrated significantly correlated PRL and LH cycles with periodicities of 33-37, 47-52, and 84-106 min. In summary, PRL release in normal young men is characterized by significant circadian and ultradian periodicities, by discrete episodic pulsations that occur approximately every 95 min, and by a close temporal coupling with LH (temporal lag between LH and PRL of 0-20 min).",
author = "Veldhuis, {Johannes D} and Johnson, {M. L.}",
year = "1988",
language = "English (US)",
volume = "67",
pages = "116--123",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
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T1 - Operating characteristics of the hypothalamo-pituitary-gonadal axis in men

T2 - Circadian, ultradian, and pulsatile release of prolactin and its temporal coupling with luteinizing hormone

AU - Veldhuis, Johannes D

AU - Johnson, M. L.

PY - 1988

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N2 - To invetigate the circadian, ultradian, and pulsatile nature of PRL release in the human, we sampled blood at 10-min intervals for 24 h in each of 12 normal young men. The subsequent serum PRL time series were subjected to contemporary techniques of rhythmic and episodic peak detection. Fourier analysis revealed a significant circadian rhythm in serum PRL concentrations in all 12 men. The mean circadian amplitude was 2.1 ± 0.3 ng/mL (μg/L), which accounted for an average of 30 ± 4% of the 24-h mean PRL concentration. In addition, multiple ultradian PRL rhythms were found with periodicities ranging from 22-242 min. Spectral analysis disclosed ultradian cycles with periodicities of 30-32, 51-59, 90-98, and 234 min. Assessment of episodic PRL pulsatility by Cluster analysis revealed 14 ± 1 PRL peaks/24 h (P < 0.01 vs. signal-free noise), which occurred at an interpulse interval of 95 ± 6 min. The average duration of a serum PRL peak was 67 ± 5 min, and its incremental amplitude was 4.0 ± 0.3 ng/ml (μg/L), which represented a 58 ± 6% increase above the preceding nadir. Discrete PRL peaks were separated by nonpulsatile valleys, with a mean duration of 27 ± 1 min. Analysis of the temporal coupling between LH and PRL release by bivariate autoregressive modeling in six men revealed significant cross-correlations between LH and simultaneous PRL concentrations as well as between LH and PRL concentrations that lagged LH by 10 or 20 min. Cross-spectral analysis demonstrated significantly correlated PRL and LH cycles with periodicities of 33-37, 47-52, and 84-106 min. In summary, PRL release in normal young men is characterized by significant circadian and ultradian periodicities, by discrete episodic pulsations that occur approximately every 95 min, and by a close temporal coupling with LH (temporal lag between LH and PRL of 0-20 min).

AB - To invetigate the circadian, ultradian, and pulsatile nature of PRL release in the human, we sampled blood at 10-min intervals for 24 h in each of 12 normal young men. The subsequent serum PRL time series were subjected to contemporary techniques of rhythmic and episodic peak detection. Fourier analysis revealed a significant circadian rhythm in serum PRL concentrations in all 12 men. The mean circadian amplitude was 2.1 ± 0.3 ng/mL (μg/L), which accounted for an average of 30 ± 4% of the 24-h mean PRL concentration. In addition, multiple ultradian PRL rhythms were found with periodicities ranging from 22-242 min. Spectral analysis disclosed ultradian cycles with periodicities of 30-32, 51-59, 90-98, and 234 min. Assessment of episodic PRL pulsatility by Cluster analysis revealed 14 ± 1 PRL peaks/24 h (P < 0.01 vs. signal-free noise), which occurred at an interpulse interval of 95 ± 6 min. The average duration of a serum PRL peak was 67 ± 5 min, and its incremental amplitude was 4.0 ± 0.3 ng/ml (μg/L), which represented a 58 ± 6% increase above the preceding nadir. Discrete PRL peaks were separated by nonpulsatile valleys, with a mean duration of 27 ± 1 min. Analysis of the temporal coupling between LH and PRL release by bivariate autoregressive modeling in six men revealed significant cross-correlations between LH and simultaneous PRL concentrations as well as between LH and PRL concentrations that lagged LH by 10 or 20 min. Cross-spectral analysis demonstrated significantly correlated PRL and LH cycles with periodicities of 33-37, 47-52, and 84-106 min. In summary, PRL release in normal young men is characterized by significant circadian and ultradian periodicities, by discrete episodic pulsations that occur approximately every 95 min, and by a close temporal coupling with LH (temporal lag between LH and PRL of 0-20 min).

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