Oncolytic measles virus retargeting by ligand display

Pavlos Msaouel, Ianko D. Iankov, Cory Allen, Stephen J Russell, Evanthia Galanis

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Despite significant advances in recent years, treatment of metastatic malignancies remains a significant challenge. There is an urgent need for development of novel therapeutic approaches. Virotherapy approaches have considerable potential, and among them measles virus (MV) vaccine strains have emerged as a promising oncolytic platform. Retargeted MV strains deriving from the Edmonston vaccine lineage (MV-Edm) have shown comparable antitumor efficacy to unmodified strains against receptor expressing tumor cells with improved therapeutic index. Here, we describe the construction, rescue, amplification, and titration of fully retargeted MV-Edm derivatives displaying tumor specific receptor binding ligands on the viral surface in combination with H protein CD46 and SLAM entry ablating mutations.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages141-162
Number of pages22
Volume797
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume797
ISSN (Print)10643745

Fingerprint

Oncolytic Viruses
Measles virus
Ligands
Measles Vaccine
Neoplasms
Vaccines
Mutation
Therapeutics

Keywords

  • Measles engineering
  • Measles retargeting
  • Oncolytic measles virus
  • Virotherapy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Msaouel, P., Iankov, I. D., Allen, C., Russell, S. J., & Galanis, E. (2012). Oncolytic measles virus retargeting by ligand display. In Methods in Molecular Biology (Vol. 797, pp. 141-162). (Methods in Molecular Biology; Vol. 797). https://doi.org/10.1007/978-1-61779-340-0_11

Oncolytic measles virus retargeting by ligand display. / Msaouel, Pavlos; Iankov, Ianko D.; Allen, Cory; Russell, Stephen J; Galanis, Evanthia.

Methods in Molecular Biology. Vol. 797 2012. p. 141-162 (Methods in Molecular Biology; Vol. 797).

Research output: Chapter in Book/Report/Conference proceedingChapter

Msaouel, P, Iankov, ID, Allen, C, Russell, SJ & Galanis, E 2012, Oncolytic measles virus retargeting by ligand display. in Methods in Molecular Biology. vol. 797, Methods in Molecular Biology, vol. 797, pp. 141-162. https://doi.org/10.1007/978-1-61779-340-0_11
Msaouel P, Iankov ID, Allen C, Russell SJ, Galanis E. Oncolytic measles virus retargeting by ligand display. In Methods in Molecular Biology. Vol. 797. 2012. p. 141-162. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-340-0_11
Msaouel, Pavlos ; Iankov, Ianko D. ; Allen, Cory ; Russell, Stephen J ; Galanis, Evanthia. / Oncolytic measles virus retargeting by ligand display. Methods in Molecular Biology. Vol. 797 2012. pp. 141-162 (Methods in Molecular Biology).
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