Oncogene expression in T-cell lymphoproliferative disorders

Gayle E. Woloschak, W. Craig Hooper, Mary J. Doerge, Robert L. Phyliky, Thomas E. Witzig, Peter M. Banks, Gordon W. Dewald, Chin Yang Li

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We have investigated the expression of oncogenes and other related genes in eleven patients with T-cell lymphoproliferative disorders and ten patients with other hematologic malignancies. The phenotypes of the T-cell disorders were determined using monoclonal antibodies specific for helper or suppressor subsets. RNA preparations were isolated from peripheral blood mononuclear cells and/ or lymph node sections, 5′-end labeled with γ-32P-ATP, and hybridized under stringent conditions to an excess of nitrocellulose-bound specific cloned DNA; autoradiographs were analysed by microdensitometry. Results revealed increased expression of K-ras, v-fps, transferrin receptor, α-tubulin and α-interferon in at least five of six helper T-cell lymphoproliferative disorders, while five of five suppressor T-cell disorders demonstrated levels of hybridization to these clones no higher than background. However, studies of T-suppressor disorders demonstrated enhanced levels of β-interferon-specific RNA in five of five patients, an increase apparent in three of six T-helper chronic lymphoproliferative disorders. These results demonstrate different patterns of gene expression evident in T-helper and T-suppressor abnormalities.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalLeukemia Research
Volume12
Issue number4
DOIs
StatePublished - 1988

Keywords

  • Oncogenes
  • T-cells
  • gene expression
  • interferon
  • leukemia
  • lymphoma
  • lymphoproliferation

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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  • Cite this

    Woloschak, G. E., Hooper, W. C., Doerge, M. J., Phyliky, R. L., Witzig, T. E., Banks, P. M., Dewald, G. W., & Li, C. Y. (1988). Oncogene expression in T-cell lymphoproliferative disorders. Leukemia Research, 12(4), 327-337. https://doi.org/10.1016/0145-2126(88)90048-3