Abstract
We have investigated the expression of oncogenes and other related genes in eleven patients with T-cell lymphoproliferative disorders and ten patients with other hematologic malignancies. The phenotypes of the T-cell disorders were determined using monoclonal antibodies specific for helper or suppressor subsets. RNA preparations were isolated from peripheral blood mononuclear cells and/ or lymph node sections, 5′-end labeled with γ-32P-ATP, and hybridized under stringent conditions to an excess of nitrocellulose-bound specific cloned DNA; autoradiographs were analysed by microdensitometry. Results revealed increased expression of K-ras, v-fps, transferrin receptor, α-tubulin and α-interferon in at least five of six helper T-cell lymphoproliferative disorders, while five of five suppressor T-cell disorders demonstrated levels of hybridization to these clones no higher than background. However, studies of T-suppressor disorders demonstrated enhanced levels of β-interferon-specific RNA in five of five patients, an increase apparent in three of six T-helper chronic lymphoproliferative disorders. These results demonstrate different patterns of gene expression evident in T-helper and T-suppressor abnormalities.
Original language | English (US) |
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Pages (from-to) | 327-337 |
Number of pages | 11 |
Journal | Leukemia Research |
Volume | 12 |
Issue number | 4 |
DOIs | |
State | Published - 1988 |
Keywords
- Oncogenes
- T-cells
- gene expression
- interferon
- leukemia
- lymphoma
- lymphoproliferation
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research