Oncogene expression in T-cell lymphoproliferative disorders

Gayle E. Woloschak, W. Craig Hooper, Mary J. Doerge, Robert L. Phyliky, Thomas Elmer Witzig, Peter M. Banks, Gordon W. Dewald, Chin Yang Li

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have investigated the expression of oncogenes and other related genes in eleven patients with T-cell lymphoproliferative disorders and ten patients with other hematologic malignancies. The phenotypes of the T-cell disorders were determined using monoclonal antibodies specific for helper or suppressor subsets. RNA preparations were isolated from peripheral blood mononuclear cells and/ or lymph node sections, 5′-end labeled with γ-32P-ATP, and hybridized under stringent conditions to an excess of nitrocellulose-bound specific cloned DNA; autoradiographs were analysed by microdensitometry. Results revealed increased expression of K-ras, v-fps, transferrin receptor, α-tubulin and α-interferon in at least five of six helper T-cell lymphoproliferative disorders, while five of five suppressor T-cell disorders demonstrated levels of hybridization to these clones no higher than background. However, studies of T-suppressor disorders demonstrated enhanced levels of β-interferon-specific RNA in five of five patients, an increase apparent in three of six T-helper chronic lymphoproliferative disorders. These results demonstrate different patterns of gene expression evident in T-helper and T-suppressor abnormalities.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalLeukemia Research
Volume12
Issue number4
DOIs
StatePublished - 1988

Fingerprint

Lymphoproliferative Disorders
Oncogenes
T-Lymphocytes
Interferons
RNA
Transferrin Receptors
Collodion
Hematologic Neoplasms
Tubulin
Helper-Inducer T-Lymphocytes
Blood Cells
Clone Cells
Adenosine Triphosphate
Lymph Nodes
Monoclonal Antibodies
Phenotype
Gene Expression
DNA
Genes

Keywords

  • gene expression
  • interferon
  • leukemia
  • lymphoma
  • lymphoproliferation
  • Oncogenes
  • T-cells

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Woloschak, G. E., Hooper, W. C., Doerge, M. J., Phyliky, R. L., Witzig, T. E., Banks, P. M., ... Li, C. Y. (1988). Oncogene expression in T-cell lymphoproliferative disorders. Leukemia Research, 12(4), 327-337. https://doi.org/10.1016/0145-2126(88)90048-3

Oncogene expression in T-cell lymphoproliferative disorders. / Woloschak, Gayle E.; Hooper, W. Craig; Doerge, Mary J.; Phyliky, Robert L.; Witzig, Thomas Elmer; Banks, Peter M.; Dewald, Gordon W.; Li, Chin Yang.

In: Leukemia Research, Vol. 12, No. 4, 1988, p. 327-337.

Research output: Contribution to journalArticle

Woloschak, GE, Hooper, WC, Doerge, MJ, Phyliky, RL, Witzig, TE, Banks, PM, Dewald, GW & Li, CY 1988, 'Oncogene expression in T-cell lymphoproliferative disorders', Leukemia Research, vol. 12, no. 4, pp. 327-337. https://doi.org/10.1016/0145-2126(88)90048-3
Woloschak GE, Hooper WC, Doerge MJ, Phyliky RL, Witzig TE, Banks PM et al. Oncogene expression in T-cell lymphoproliferative disorders. Leukemia Research. 1988;12(4):327-337. https://doi.org/10.1016/0145-2126(88)90048-3
Woloschak, Gayle E. ; Hooper, W. Craig ; Doerge, Mary J. ; Phyliky, Robert L. ; Witzig, Thomas Elmer ; Banks, Peter M. ; Dewald, Gordon W. ; Li, Chin Yang. / Oncogene expression in T-cell lymphoproliferative disorders. In: Leukemia Research. 1988 ; Vol. 12, No. 4. pp. 327-337.
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