Oligonucleotide array CGH studies in myeloproliferative neoplasms: Comparison with JAK2V617F mutational status and conventional chromosome analysis

Ayalew Tefferi, Shireen Sirhan, Yi Sun, Terra Lasho, Christy M. Finke, James Weisberger, Sherri Bale, John Compton, Charles A. LeDuc, Animesh Pardanani, Erik C. Thorland, Yuriy Shevchenko, Marc Grodman, Wendy K. Chung

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Comparative genomic hybridization (CGH), using oligo arrays with either 44,000 or 105,000 oligonucleotides, was performed on granulocyte-derived DNA from 71 patients with BCR-ABL-negative classic myeloproliferative neoplasms (MPNs): 32 primary myelofibrosis (PMF), 26 polycythemia vera (PV) and 13 essential thrombocythemia (ET). Copy number changes (CNCs) were detected in 44%, 35%, and 15% of the cases with PMF, PV and ET, respectively. In ET and PMF, CNCs were more frequently detected in the presence of JAK2V617F (50% vs. 19%; p = 0.05). Conventional chromosome analysis was obtained in 57 patients either at diagnosis or within 1 year of the array CGH study; all 21 patients with PV and 11 with ET displayed normal cytogenetic findings despite the presence of CNCs in 29% and 18%, respectively. In PMF, the respective rates of CNCs and abnormal karyotype were 48% and 36%; karyotypic abnormalities, including unbalanced translocations, were often detected by array CGH as chromosomal gains or losses. This preliminary report suggests a potential value for array CGH in terms of both clinical diagnostics and genomic research in MPNs.

Original languageEnglish (US)
Pages (from-to)662-664
Number of pages3
JournalLeukemia Research
Volume33
Issue number5
DOIs
StatePublished - May 1 2009

Keywords

  • Array CGH
  • JAK2 V617F
  • Myelofibrosis
  • Myeloproliferative
  • Polycythemia
  • Thrombocythemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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