Oligodendroglioma cell lines containing t(1;19)(q10;p10)

John J P Kelly, Michael D. Blough, Owen D M Stechishin, Jennifer A W Chan, Desiree Beauchamp, Marco Perizzolo, Doug J. Demetrick, Lisa Steele, Roland N. Auer, Walter J. Hader, Morgan Westgate, Ian F Parney, Robert Brian Jenkins, J. Gregory Cairncross, Samuel Weiss

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Investigating the biology of oligodendroglioma and its characteristic combined deletion of chromosomal arms 1p and 19q, mediated by an unbalanced translocation, t(1;19)(q10;p10), has been hampered by the lack of cell lines that harbor these traits. We grew cells from 2 anaplastic oligodendrogliomas in serum-free conditions. Serial propagation and expansion led to the establishment of permanent cell lines that maintained the genetic signature of the parent oligodendrogliomas and displayed features of brain tumor stem cells in vitro. One line was established from a treatment-naïve tumor and the other from a temozolomide resistant recurrent tumor. These lines may be important tools for understanding the biology of oligodendrogliomas and the function of their defining genetic traits.

Original languageEnglish (US)
Pages (from-to)745-755
Number of pages11
JournalNeuro-Oncology
Volume12
Issue number7
DOIs
StatePublished - Jul 2010

Fingerprint

Oligodendroglioma
Cell Line
temozolomide
Neoplastic Stem Cells
Brain Neoplasms
Neoplasms
Serum

Keywords

  • 19q
  • 1p
  • Brain tumor stem cell
  • IDH1
  • Oligodendroglioma
  • Translocation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Kelly, J. J. P., Blough, M. D., Stechishin, O. D. M., Chan, J. A. W., Beauchamp, D., Perizzolo, M., ... Weiss, S. (2010). Oligodendroglioma cell lines containing t(1;19)(q10;p10). Neuro-Oncology, 12(7), 745-755. https://doi.org/10.1093/neuonc/noq031

Oligodendroglioma cell lines containing t(1;19)(q10;p10). / Kelly, John J P; Blough, Michael D.; Stechishin, Owen D M; Chan, Jennifer A W; Beauchamp, Desiree; Perizzolo, Marco; Demetrick, Doug J.; Steele, Lisa; Auer, Roland N.; Hader, Walter J.; Westgate, Morgan; Parney, Ian F; Jenkins, Robert Brian; Cairncross, J. Gregory; Weiss, Samuel.

In: Neuro-Oncology, Vol. 12, No. 7, 07.2010, p. 745-755.

Research output: Contribution to journalArticle

Kelly, JJP, Blough, MD, Stechishin, ODM, Chan, JAW, Beauchamp, D, Perizzolo, M, Demetrick, DJ, Steele, L, Auer, RN, Hader, WJ, Westgate, M, Parney, IF, Jenkins, RB, Cairncross, JG & Weiss, S 2010, 'Oligodendroglioma cell lines containing t(1;19)(q10;p10)', Neuro-Oncology, vol. 12, no. 7, pp. 745-755. https://doi.org/10.1093/neuonc/noq031
Kelly JJP, Blough MD, Stechishin ODM, Chan JAW, Beauchamp D, Perizzolo M et al. Oligodendroglioma cell lines containing t(1;19)(q10;p10). Neuro-Oncology. 2010 Jul;12(7):745-755. https://doi.org/10.1093/neuonc/noq031
Kelly, John J P ; Blough, Michael D. ; Stechishin, Owen D M ; Chan, Jennifer A W ; Beauchamp, Desiree ; Perizzolo, Marco ; Demetrick, Doug J. ; Steele, Lisa ; Auer, Roland N. ; Hader, Walter J. ; Westgate, Morgan ; Parney, Ian F ; Jenkins, Robert Brian ; Cairncross, J. Gregory ; Weiss, Samuel. / Oligodendroglioma cell lines containing t(1;19)(q10;p10). In: Neuro-Oncology. 2010 ; Vol. 12, No. 7. pp. 745-755.
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