Oligodendroglioma cell lines containing t(1;19)(q10;p10)

John J.P. Kelly, Michael D. Blough, Owen D.M. Stechishin, Jennifer A.W. Chan, Desiree Beauchamp, Marco Perizzolo, Doug J. Demetrick, Lisa Steele, Roland N. Auer, Walter J. Hader, Morgan Westgate, Ian F. Parney, Robert Jenkins, J. Gregory Cairncross, Samuel Weiss

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Investigating the biology of oligodendroglioma and its characteristic combined deletion of chromosomal arms 1p and 19q, mediated by an unbalanced translocation, t(1;19)(q10;p10), has been hampered by the lack of cell lines that harbor these traits. We grew cells from 2 anaplastic oligodendrogliomas in serum-free conditions. Serial propagation and expansion led to the establishment of permanent cell lines that maintained the genetic signature of the parent oligodendrogliomas and displayed features of brain tumor stem cells in vitro. One line was established from a treatment-naïve tumor and the other from a temozolomide resistant recurrent tumor. These lines may be important tools for understanding the biology of oligodendrogliomas and the function of their defining genetic traits.

Original languageEnglish (US)
Pages (from-to)745-755
Number of pages11
JournalNeuro-oncology
Volume12
Issue number7
DOIs
StatePublished - Jul 2010

Keywords

  • 19q
  • 1p
  • Brain tumor stem cell
  • IDH1
  • Oligodendroglioma
  • Translocation

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

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