Oligodendroglioma cell lines containing t(1;19)(q10;p10)

John J.P. Kelly; Michael D. Blough; Owen D.M. Stechishin; Jennifer A.W. Chan; Desiree Beauchamp; Marco Perizzolo; Doug J. Demetrick; Lisa Steele; Roland N. Auer; Walter J. Hader; Morgan Westgate; Ian F. Parney; Robert Jenkins; J. Gregory Cairncross; Samuel Weiss

doi:10.1093/neuonc/noq031

Oligodendroglioma cell lines containing t(1;19)(q10;p10)

John J.P. Kelly, Michael D. Blough, Owen D.M. Stechishin, Jennifer A.W. Chan, Desiree Beauchamp, Marco Perizzolo, Doug J. Demetrick, Lisa Steele, Roland N. Auer, Walter J. Hader, Morgan Westgate, Ian F. Parney, Robert Jenkins, J. Gregory Cairncross, Samuel Weiss

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Investigating the biology of oligodendroglioma and its characteristic combined deletion of chromosomal arms 1p and 19q, mediated by an unbalanced translocation, t(1;19)(q10;p10), has been hampered by the lack of cell lines that harbor these traits. We grew cells from 2 anaplastic oligodendrogliomas in serum-free conditions. Serial propagation and expansion led to the establishment of permanent cell lines that maintained the genetic signature of the parent oligodendrogliomas and displayed features of brain tumor stem cells in vitro. One line was established from a treatment-naïve tumor and the other from a temozolomide resistant recurrent tumor. These lines may be important tools for understanding the biology of oligodendrogliomas and the function of their defining genetic traits.

Original language	English (US)
Pages (from-to)	745-755
Number of pages	11
Journal	Neuro-oncology
Volume	12
Issue number	7
DOIs	https://doi.org/10.1093/neuonc/noq031
State	Published - Jul 2010

Keywords

19q
1p
Brain tumor stem cell
IDH1
Oligodendroglioma
Translocation

ASJC Scopus subject areas

Oncology
Clinical Neurology
Cancer Research

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10.1093/neuonc/noq031

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title = "Oligodendroglioma cell lines containing t(1;19)(q10;p10)",

abstract = "Investigating the biology of oligodendroglioma and its characteristic combined deletion of chromosomal arms 1p and 19q, mediated by an unbalanced translocation, t(1;19)(q10;p10), has been hampered by the lack of cell lines that harbor these traits. We grew cells from 2 anaplastic oligodendrogliomas in serum-free conditions. Serial propagation and expansion led to the establishment of permanent cell lines that maintained the genetic signature of the parent oligodendrogliomas and displayed features of brain tumor stem cells in vitro. One line was established from a treatment-na{\"i}ve tumor and the other from a temozolomide resistant recurrent tumor. These lines may be important tools for understanding the biology of oligodendrogliomas and the function of their defining genetic traits.",

keywords = "19q, 1p, Brain tumor stem cell, IDH1, Oligodendroglioma, Translocation",

author = "Kelly, {John J.P.} and Blough, {Michael D.} and Stechishin, {Owen D.M.} and Chan, {Jennifer A.W.} and Desiree Beauchamp and Marco Perizzolo and Demetrick, {Doug J.} and Lisa Steele and Auer, {Roland N.} and Hader, {Walter J.} and Morgan Westgate and Parney, {Ian F.} and Robert Jenkins and Cairncross, {J. Gregory} and Samuel Weiss",

year = "2010",

month = jul,

doi = "10.1093/neuonc/noq031",

language = "English (US)",

volume = "12",

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journal = "Neuro-oncology",

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AU - Blough, Michael D.

AU - Stechishin, Owen D.M.

AU - Chan, Jennifer A.W.

AU - Beauchamp, Desiree

AU - Perizzolo, Marco

AU - Demetrick, Doug J.

AU - Steele, Lisa

AU - Auer, Roland N.

AU - Hader, Walter J.

AU - Westgate, Morgan

AU - Parney, Ian F.

AU - Jenkins, Robert

AU - Cairncross, J. Gregory

AU - Weiss, Samuel

PY - 2010/7

Y1 - 2010/7

N2 - Investigating the biology of oligodendroglioma and its characteristic combined deletion of chromosomal arms 1p and 19q, mediated by an unbalanced translocation, t(1;19)(q10;p10), has been hampered by the lack of cell lines that harbor these traits. We grew cells from 2 anaplastic oligodendrogliomas in serum-free conditions. Serial propagation and expansion led to the establishment of permanent cell lines that maintained the genetic signature of the parent oligodendrogliomas and displayed features of brain tumor stem cells in vitro. One line was established from a treatment-naïve tumor and the other from a temozolomide resistant recurrent tumor. These lines may be important tools for understanding the biology of oligodendrogliomas and the function of their defining genetic traits.

AB - Investigating the biology of oligodendroglioma and its characteristic combined deletion of chromosomal arms 1p and 19q, mediated by an unbalanced translocation, t(1;19)(q10;p10), has been hampered by the lack of cell lines that harbor these traits. We grew cells from 2 anaplastic oligodendrogliomas in serum-free conditions. Serial propagation and expansion led to the establishment of permanent cell lines that maintained the genetic signature of the parent oligodendrogliomas and displayed features of brain tumor stem cells in vitro. One line was established from a treatment-naïve tumor and the other from a temozolomide resistant recurrent tumor. These lines may be important tools for understanding the biology of oligodendrogliomas and the function of their defining genetic traits.

KW - 19q

KW - 1p

KW - Brain tumor stem cell

KW - IDH1

KW - Oligodendroglioma

KW - Translocation

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Oligodendroglioma cell lines containing t(1;19)(q10;p10)

Abstract

Keywords

ASJC Scopus subject areas

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