Oleate-induced formation of fat cells with impaired insulin sensitivity

Weisheng Xie, James A. Hamilton, James L Kirkland, Barbara E. Corkey, Wen Guo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Exogenous FA cause lipid accumulation in preadipocytes. We investigated whether the fat cells thus formed are metabolically distinct from adipocytes differentiated with standard methylisobutylxanthine, dexamethasone, and insulin (MDI) hormonal cocktail by comparing their expression of adipogenic genes, accumulation of TAG, lipogenesis, lipolysis, glucose uptake, and the effects of insulin on selected metabolic activities. Cells exposed to oleate began to accumulate TAG in parallel or prior to the induction of adipogenic genes, whereas cells treated with MDI expressed adipogenic genes before TAG accumulation. Oleate-treated fat cells also showed exaggerated basal lipolysis and weak response to insulin in both lipolysis regulation and glucose uptake. These findings were associated with increased basal phosphorylation of perilipin, increased Glut-1 but decreased Glut-4 expression, and reduced insulin-induced Akt phosphorylation. We suggest that this unique fat cell phenotype might be a mimetic of what can happen to fat cells formed in vivo under the influence of circulating FA and might be a useful model for in vitro studies of obesity-related insulin resistance in adipocytes.

Original languageEnglish (US)
Pages (from-to)267-271
Number of pages5
JournalLipids
Volume41
Issue number3
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

Oleic Acid
adipocytes
Adipocytes
insulin resistance
oleic acid
Insulin Resistance
Fats
Cells
Insulin
insulin
Lipolysis
lipolysis
Phosphorylation
Genes
dexamethasone
Dexamethasone
phosphorylation
uptake mechanisms
Glucose
Lipogenesis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Biochemistry

Cite this

Oleate-induced formation of fat cells with impaired insulin sensitivity. / Xie, Weisheng; Hamilton, James A.; Kirkland, James L; Corkey, Barbara E.; Guo, Wen.

In: Lipids, Vol. 41, No. 3, 05.2006, p. 267-271.

Research output: Contribution to journalArticle

Xie, Weisheng ; Hamilton, James A. ; Kirkland, James L ; Corkey, Barbara E. ; Guo, Wen. / Oleate-induced formation of fat cells with impaired insulin sensitivity. In: Lipids. 2006 ; Vol. 41, No. 3. pp. 267-271.
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