Octamerization enables soluble CD46 receptor to neutralize measles virus in vitro and in vivo

Dale Christiansen, Patricia Devaux, Brigitte Réveil, Alexey Evlashev, Branka Horvat, Josette Lamy, Chantal Rabourdin-Combe, Jacques H.M. Cohen, Denis Gerlier

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

A chimeric fusion protein encompassing the CD46 ectodomain linked to the C-terminal part of the C4b binding protein (C4bp) α chain (sCD46-C4bpα) was produced in eukaryotic cells. This protein, secreted as a disulfide-linked homo-octamer, was recognized by a panel of anti-CD46 antibodies with varying avidities. Unlike monomeric sCB46, the octameric sCD46-C4bpα protein was devoid of complement regulatory activity. However, sCB46-C4bpα was able to bind to the measles virus hemagglutinin protein expressed on murine cells with a higher avidity than soluble monomeric sCD46. Moreover, the octameric sCD46-C4bpα protein was significantly more efficient than monomeric sCD45 in inhibiting virus binding to CD46, in blocking virus induced cell-cell fusion, and in neutralizing measles virus in vitro. In addition, the octameric sCD46- C4bpα protein, but not the monameric sCD46, fully protected CD46 transgenic mice against a lethal intracranial measles virus challenge.

Original languageEnglish (US)
Pages (from-to)4672-4678
Number of pages7
JournalJournal of virology
Volume74
Issue number10
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Octamerization enables soluble CD46 receptor to neutralize measles virus in vitro and in vivo'. Together they form a unique fingerprint.

  • Cite this

    Christiansen, D., Devaux, P., Réveil, B., Evlashev, A., Horvat, B., Lamy, J., Rabourdin-Combe, C., Cohen, J. H. M., & Gerlier, D. (2000). Octamerization enables soluble CD46 receptor to neutralize measles virus in vitro and in vivo. Journal of virology, 74(10), 4672-4678. https://doi.org/10.1128/JVI.74.10.4672-4678.2000