TY - JOUR
T1 - Occupancy of an adhesive glycoprotein receptor modulates expression of an antigenic site involved in cell adhesion
AU - Frelinger, A. L.
AU - Lam, S. C.T.
AU - Plow, E. F.
AU - Smith, M. A.
AU - Loftus, J. C.
AU - Ginsberg, M. H.
PY - 1988
Y1 - 1988
N2 - Binding of ligands that contain Arg-Gly-Asp to adhesion receptors induces cell spreading and aggregation and alters gene expression, possibly due to conformational changes within occupied adhesion receptors. PMI-1 is a monoclonal antibody which reacts with the platelet fibrinogen receptor, glycoprotein IIb-IIIa, and reports such a conformational change. ADP stimulation of platelets results in a fibrinogen-dependent increase in binding of the PMI-1 antibody. Peptides containing Arg-Gly-Asp also reversibly increase the binding of this antibody to cells and to purified glycoprotein IIb-IIIa. The PMI-1 antibody inhibits platelet adhesion and spreading on certain substrata (Shadle, P.J., Ginsberg, M.H., Plow, E.F., and Barondes, S.H. (1984) J. Cell Biol. 99, 2056-2060); thus this occupancy-modulated site may participate in adhesive function.
AB - Binding of ligands that contain Arg-Gly-Asp to adhesion receptors induces cell spreading and aggregation and alters gene expression, possibly due to conformational changes within occupied adhesion receptors. PMI-1 is a monoclonal antibody which reacts with the platelet fibrinogen receptor, glycoprotein IIb-IIIa, and reports such a conformational change. ADP stimulation of platelets results in a fibrinogen-dependent increase in binding of the PMI-1 antibody. Peptides containing Arg-Gly-Asp also reversibly increase the binding of this antibody to cells and to purified glycoprotein IIb-IIIa. The PMI-1 antibody inhibits platelet adhesion and spreading on certain substrata (Shadle, P.J., Ginsberg, M.H., Plow, E.F., and Barondes, S.H. (1984) J. Cell Biol. 99, 2056-2060); thus this occupancy-modulated site may participate in adhesive function.
UR - http://www.scopus.com/inward/record.url?scp=0024208260&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024208260&partnerID=8YFLogxK
M3 - Article
C2 - 2457583
AN - SCOPUS:0024208260
SN - 0021-9258
VL - 263
SP - 12397
EP - 12402
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 25
ER -