Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution

L. T. Perelman; V. Backman; M. Wallace; G. Zonios; R. Manoharan; A. Nusrat; S. Shields; M. Seiler; C. Lima; T. Hamano; I. Itzkan; J. Van Dam; J. M. Crawford; M. S. Feld

doi:10.1103/PhysRevLett.80.627

Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, M. S. Feld

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

452 Scopus citations

Abstract

We report observation of a fine structure component in backscattered light from mucosal tissue which is periodic in wavelength. This structure is ordinarily masked by a diffusive background. We have identified the origin of this component as being due to light which is Mie scattered by surface epithelial cell nuclei. By analyzing the amplitude and frequency of the fine structure, the density and size distribution of these nuclei can be extracted. These quantities are important indicators of neoplastic precancerous changes in biological tissue.

Original language	English (US)
Pages (from-to)	627-630
Number of pages	4
Journal	Physical Review Letters
Volume	80
Issue number	3
DOIs	https://doi.org/10.1103/PhysRevLett.80.627
State	Published - 1998

ASJC Scopus subject areas

General Physics and Astronomy

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10.1103/PhysRevLett.80.627

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Perelman, L. T., Backman, V., Wallace, M., Zonios, G., Manoharan, R., Nusrat, A., Shields, S., Seiler, M., Lima, C., Hamano, T., Itzkan, I., Van Dam, J., Crawford, J. M., & Feld, M. S. (1998). Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution. Physical Review Letters, 80(3), 627-630. https://doi.org/10.1103/PhysRevLett.80.627

Perelman, LT, Backman, V, Wallace, M, Zonios, G, Manoharan, R, Nusrat, A, Shields, S, Seiler, M, Lima, C, Hamano, T, Itzkan, I, Van Dam, J, Crawford, JM & Feld, MS 1998, 'Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution', Physical Review Letters, vol. 80, no. 3, pp. 627-630. https://doi.org/10.1103/PhysRevLett.80.627

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AU - Zonios, G.

AU - Manoharan, R.

AU - Nusrat, A.

AU - Shields, S.

AU - Seiler, M.

AU - Lima, C.

AU - Hamano, T.

AU - Itzkan, I.

AU - Van Dam, J.

AU - Crawford, J. M.

AU - Feld, M. S.

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Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution

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