Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, M. S. Feld

Research output: Contribution to journalArticle

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Abstract

We report observation of a fine structure component in backscattered light from mucosal tissue which is periodic in wavelength. This structure is ordinarily masked by a diffusive background. We have identified the origin of this component as being due to light which is Mie scattered by surface epithelial cell nuclei. By analyzing the amplitude and frequency of the fine structure, the density and size distribution of these nuclei can be extracted. These quantities are important indicators of neoplastic precancerous changes in biological tissue.

Original languageEnglish (US)
Pages (from-to)627-630
Number of pages4
JournalPhysical Review Letters
Volume80
Issue number3
DOIs
StatePublished - Jan 1 1998

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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    Perelman, L. T., Backman, V., Wallace, M., Zonios, G., Manoharan, R., Nusrat, A., Shields, S., Seiler, M., Lima, C., Hamano, T., Itzkan, I., Van Dam, J., Crawford, J. M., & Feld, M. S. (1998). Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution. Physical Review Letters, 80(3), 627-630. https://doi.org/10.1103/PhysRevLett.80.627