Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution

L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, M. S. Feld

Research output: Contribution to journalArticle

442 Citations (Scopus)

Abstract

We report observation of a fine structure component in backscattcred light from mucosal tissue which is periodic in wavelength. This structure is ordinarily masked by a diffusive background. We have identified the origin of this component as being due to light which is Mie scattered by surface epithelial cell nuclei. By analyzing the amplitude and frequency of the fine structure, the density and size distribution of these nuclei can be extracted. These quantities are important indicators of neoplastic precancerous changes in biological tissue.

Original languageEnglish (US)
Pages (from-to)627-630
Number of pages4
JournalPhysical Review Letters
Volume80
Issue number3
StatePublished - 1998
Externally publishedYes

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fine structure
reflectance
nuclei
density distribution
wavelengths

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Perelman, L. T., Backman, V., Wallace, M., Zonios, G., Manoharan, R., Nusrat, A., ... Feld, M. S. (1998). Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution. Physical Review Letters, 80(3), 627-630.

Observation of periodic fine structure in reflectance from biological tissue : A new technique for measuring nuclear size distribution. / Perelman, L. T.; Backman, V.; Wallace, M.; Zonios, G.; Manoharan, R.; Nusrat, A.; Shields, S.; Seiler, M.; Lima, C.; Hamano, T.; Itzkan, I.; Van Dam, J.; Crawford, J. M.; Feld, M. S.

In: Physical Review Letters, Vol. 80, No. 3, 1998, p. 627-630.

Research output: Contribution to journalArticle

Perelman, LT, Backman, V, Wallace, M, Zonios, G, Manoharan, R, Nusrat, A, Shields, S, Seiler, M, Lima, C, Hamano, T, Itzkan, I, Van Dam, J, Crawford, JM & Feld, MS 1998, 'Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution', Physical Review Letters, vol. 80, no. 3, pp. 627-630.
Perelman, L. T. ; Backman, V. ; Wallace, M. ; Zonios, G. ; Manoharan, R. ; Nusrat, A. ; Shields, S. ; Seiler, M. ; Lima, C. ; Hamano, T. ; Itzkan, I. ; Van Dam, J. ; Crawford, J. M. ; Feld, M. S. / Observation of periodic fine structure in reflectance from biological tissue : A new technique for measuring nuclear size distribution. In: Physical Review Letters. 1998 ; Vol. 80, No. 3. pp. 627-630.
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