Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells

Mihir Bagchi, Shamim A. Ansari, Danielle M. Lindenmuth, Andre J van Wijnen, Jane Lian, Janet L. Stein, Gary S. Stein

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Association of transcription factors with the nuclear matrix represents a mechanism by which nuclear architecture may influence transcriptional control of gene expression. This investigation examines nuclear matrix associated proteins (NMP's) isolated from ocular lens epithelial cells by monitoring DNA binding activities using consensus oligonucleotides recognized by the transcription factors YY1, AML-1, AP-1, SP-1 and ATF. The nuclear matrix fractions tested included an immortilized human lens epithelial cell line containing the SV40 large T-antigen, and two mouse lens epithelial cell lines derived from either a normal mouse or a cataract mouse. A rabbit epidermal epithelial cell line and HeLa cells were also included in this study for comparison. The data from these experiments reveal that ubiquitously represented and tissue restricted regulatory proteins are associated with nuclear matrix of lens epithelial cells. The functional significance of the nuclear matrix association of these transcription factors remains to be determined. However, our findings raise the possibility that the transcription factors associated with the nuclear matrix could have specific roles in gene regulation and eye tissue development.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalMolecular Biology Reports
Volume25
Issue number1
DOIs
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Nuclear Matrix
Crystalline Lens
DNA-Binding Proteins
Lenses
Epithelial Cells
Nuclear Matrix-Associated Proteins
Transcription Factors
Gene expression
Cell Line
YY1 Transcription Factor
Tissue
Polyomavirus Transforming Antigens
Viral Tumor Antigens
Transcription Factor AP-1
HeLa Cells
Oligonucleotides
Cataract
Rabbits
Gene Expression
Monitoring

Keywords

  • Gene expression
  • Nuclear matrix proteins
  • Ocular lens epithelial cells
  • Transcription factors

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

Cite this

Bagchi, M., Ansari, S. A., Lindenmuth, D. M., van Wijnen, A. J., Lian, J., Stein, J. L., & Stein, G. S. (1998). Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells. Molecular Biology Reports, 25(1), 13-19. https://doi.org/10.1023/A:1006886110771

Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells. / Bagchi, Mihir; Ansari, Shamim A.; Lindenmuth, Danielle M.; van Wijnen, Andre J; Lian, Jane; Stein, Janet L.; Stein, Gary S.

In: Molecular Biology Reports, Vol. 25, No. 1, 01.1998, p. 13-19.

Research output: Contribution to journalArticle

Bagchi, M, Ansari, SA, Lindenmuth, DM, van Wijnen, AJ, Lian, J, Stein, JL & Stein, GS 1998, 'Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells', Molecular Biology Reports, vol. 25, no. 1, pp. 13-19. https://doi.org/10.1023/A:1006886110771
Bagchi M, Ansari SA, Lindenmuth DM, van Wijnen AJ, Lian J, Stein JL et al. Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells. Molecular Biology Reports. 1998 Jan;25(1):13-19. https://doi.org/10.1023/A:1006886110771
Bagchi, Mihir ; Ansari, Shamim A. ; Lindenmuth, Danielle M. ; van Wijnen, Andre J ; Lian, Jane ; Stein, Janet L. ; Stein, Gary S. / Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells. In: Molecular Biology Reports. 1998 ; Vol. 25, No. 1. pp. 13-19.
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