Nuclear Export of the NF-κB Inhibitor IκBα Is Required for Proper B Cell and Secondary Lymphoid Tissue Formation

Shelly M. Wuerzberger-Davis, Yuhong Chen, David T. Yang, Jeffrey D. Kearns, Paul W. Bates, Candace Lynch, Nicholas C. Ladell, Mei Yu, Andrew Podd, Hu Zeng, Tony T. Huang, Renren Wen, Alexander Hoffmann, Demin Wang, Shigeki Miyamoto

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The N-terminal nuclear export sequence (NES) of inhibitor of nuclear factor kappa B (NF-κB) alpha (IκBα) promotes NF-κB export from the cell nucleus to the cytoplasm, but the physiological role of this export regulation remains unknown. Here we report the derivation and analysis of genetically targeted mice harboring a germline mutation in IκBα NES. Mature B cells in the mutant mice displayed nuclear accumulation of inactive IκBα complexes containing a NF-κB family member, cRel, causing their spatial separation from the cytoplasmic IκB kinase. This resulted in severe reductions in constitutive and canonical NF-κB activities, synthesis of p100 and RelB NF-κB members, noncanonical NF-κB activity, NF-κB target gene induction, and proliferation and survival responses in B cells. Consequently, mice displayed defective B cell maturation, antibody production, and formation of secondary lymphoid organs and tissues. Thus, IκBα nuclear export is essential to maintain constitutive, canonical, and noncanonical NF-κB activation potentials in mature B cells in vivo.

Original languageEnglish (US)
Pages (from-to)188-200
Number of pages13
JournalImmunity
Volume34
Issue number2
DOIs
StatePublished - Feb 25 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Wuerzberger-Davis, S. M., Chen, Y., Yang, D. T., Kearns, J. D., Bates, P. W., Lynch, C., Ladell, N. C., Yu, M., Podd, A., Zeng, H., Huang, T. T., Wen, R., Hoffmann, A., Wang, D., & Miyamoto, S. (2011). Nuclear Export of the NF-κB Inhibitor IκBα Is Required for Proper B Cell and Secondary Lymphoid Tissue Formation. Immunity, 34(2), 188-200. https://doi.org/10.1016/j.immuni.2011.01.014