TY - JOUR
T1 - Nuclear Export of the NF-κB Inhibitor IκBα Is Required for Proper B Cell and Secondary Lymphoid Tissue Formation
AU - Wuerzberger-Davis, Shelly M.
AU - Chen, Yuhong
AU - Yang, David T.
AU - Kearns, Jeffrey D.
AU - Bates, Paul W.
AU - Lynch, Candace
AU - Ladell, Nicholas C.
AU - Yu, Mei
AU - Podd, Andrew
AU - Zeng, Hu
AU - Huang, Tony T.
AU - Wen, Renren
AU - Hoffmann, Alexander
AU - Wang, Demin
AU - Miyamoto, Shigeki
N1 - Funding Information:
We thank A. Griep, P. Powers, C. Bartley, and M. Lye at the Transgenic Animal Facility at the University of Wisconsin Biotechnology Center for the gift of the TK-pflox8 targeting vector and assistance with the generation of the mutant mice, B. Seufzer for genotyping of the mice, and members of the S.M., D.W., and R.W. labs for stimulating discussions. This work was supported in part by National Institutes of Health grants R01 CA081065, R56 CA081065, CA077474, and R01 GM083681 (S.M.), T32 CA009614 (D.Y.), R01 AI083453 and P01 GM071862 (A.H.), R01 AI52327 (R.W.), and R01 HL073284, R01 AI079087, and P01 HL44612 (D.W.), by UWCCC Core grant P30 CA14520 (G. Wilding, PI), and by Scholar Award from the Leukemia & Lymphoma Society (D.W.).
PY - 2011/2/25
Y1 - 2011/2/25
N2 - The N-terminal nuclear export sequence (NES) of inhibitor of nuclear factor kappa B (NF-κB) alpha (IκBα) promotes NF-κB export from the cell nucleus to the cytoplasm, but the physiological role of this export regulation remains unknown. Here we report the derivation and analysis of genetically targeted mice harboring a germline mutation in IκBα NES. Mature B cells in the mutant mice displayed nuclear accumulation of inactive IκBα complexes containing a NF-κB family member, cRel, causing their spatial separation from the cytoplasmic IκB kinase. This resulted in severe reductions in constitutive and canonical NF-κB activities, synthesis of p100 and RelB NF-κB members, noncanonical NF-κB activity, NF-κB target gene induction, and proliferation and survival responses in B cells. Consequently, mice displayed defective B cell maturation, antibody production, and formation of secondary lymphoid organs and tissues. Thus, IκBα nuclear export is essential to maintain constitutive, canonical, and noncanonical NF-κB activation potentials in mature B cells in vivo.
AB - The N-terminal nuclear export sequence (NES) of inhibitor of nuclear factor kappa B (NF-κB) alpha (IκBα) promotes NF-κB export from the cell nucleus to the cytoplasm, but the physiological role of this export regulation remains unknown. Here we report the derivation and analysis of genetically targeted mice harboring a germline mutation in IκBα NES. Mature B cells in the mutant mice displayed nuclear accumulation of inactive IκBα complexes containing a NF-κB family member, cRel, causing their spatial separation from the cytoplasmic IκB kinase. This resulted in severe reductions in constitutive and canonical NF-κB activities, synthesis of p100 and RelB NF-κB members, noncanonical NF-κB activity, NF-κB target gene induction, and proliferation and survival responses in B cells. Consequently, mice displayed defective B cell maturation, antibody production, and formation of secondary lymphoid organs and tissues. Thus, IκBα nuclear export is essential to maintain constitutive, canonical, and noncanonical NF-κB activation potentials in mature B cells in vivo.
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U2 - 10.1016/j.immuni.2011.01.014
DO - 10.1016/j.immuni.2011.01.014
M3 - Article
C2 - 21333553
AN - SCOPUS:79951768852
SN - 1074-7613
VL - 34
SP - 188
EP - 200
JO - Immunity
JF - Immunity
IS - 2
ER -