Novel porous poly(propylene fumarate-co-caprolactone) scaffolds fabricated by thermally induced phase separation

Ji Guo, Xifeng Liu, A. Lee Miller, Brian E. Waletzki, Michael J Yaszemski, Lichun Lu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Scaffolds with porous structures are highly applicable for tissue engineering and regenerative medicine. In the present study, 3-dimensional poly(propylene fumarate-co-caprolactone) [P(PF-co-CL)] scaffolds were fabricated from a P(PF-co-CL)–dioxane-water ternary system through thermally induced phase separation (TIPS). Cloud points of P(PF-co-CL) in dioxane-water solutions increased with increased solute concentration, but increased dioxane composition decreased cloud point. Among 3 polymer concentrations (4, 8, and 12 wt%), 8 wt% P(PF-co-CL) scaffolds exhibited the best pore interconnectivity, with large, regular sized pores. Scaffolds were formed in 3 solutions with different dioxane-water ratios (74/26, 78/22, and 82/18 wt/wt); the 78/22 wt/wt scaffold had finger-shaped patterns with better interconnectivity than scaffolds from the other two ratios. Higher dioxane-water ratios resulted in a larger contact angle and thus less wettability for the fabricated scaffold, while scaffolds fabricated from higher concentrations of P(PF-co-CL) or high dioxane-water ratios had better biomineralization after soaking in simulated body fluid. In vitro cell viability testing showed the scaffolds had good biocompatibility with both bone and nerve cells. The results indicate that the polymer concentration and solvents ratio significantly affect the formation of porous structures, and optimum processing parameters were found to be 8% polymer concentration and 22% to 24% water content. These porous P(PF-co-CL) scaffolds fabricated via TIPS may be useful in various tissue engineering applications

Original languageEnglish (US)
Pages (from-to)226-235
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume105
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Scaffolds
Phase separation
Polypropylenes
Fumarates
Propylene
Water
Polymers
Scaffolds (biology)
Tissue engineering
Biomineralization
poly(propylene fumarate-co-caprolactone)
Body fluids
Ternary systems
Biocompatibility
Water content
Neurons
Contact angle
Wetting
1,4-dioxane
Bone

Keywords

  • P(PF-co-CL)
  • porous scaffold
  • thermally induced phase separation
  • tissue engineering

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Novel porous poly(propylene fumarate-co-caprolactone) scaffolds fabricated by thermally induced phase separation. / Guo, Ji; Liu, Xifeng; Lee Miller, A.; Waletzki, Brian E.; Yaszemski, Michael J; Lu, Lichun.

In: Journal of Biomedical Materials Research - Part A, Vol. 105, No. 1, 01.01.2017, p. 226-235.

Research output: Contribution to journalArticle

@article{ad0e7c9b1a10431884936df5bcb23611,
title = "Novel porous poly(propylene fumarate-co-caprolactone) scaffolds fabricated by thermally induced phase separation",
abstract = "Scaffolds with porous structures are highly applicable for tissue engineering and regenerative medicine. In the present study, 3-dimensional poly(propylene fumarate-co-caprolactone) [P(PF-co-CL)] scaffolds were fabricated from a P(PF-co-CL)–dioxane-water ternary system through thermally induced phase separation (TIPS). Cloud points of P(PF-co-CL) in dioxane-water solutions increased with increased solute concentration, but increased dioxane composition decreased cloud point. Among 3 polymer concentrations (4, 8, and 12 wt{\%}), 8 wt{\%} P(PF-co-CL) scaffolds exhibited the best pore interconnectivity, with large, regular sized pores. Scaffolds were formed in 3 solutions with different dioxane-water ratios (74/26, 78/22, and 82/18 wt/wt); the 78/22 wt/wt scaffold had finger-shaped patterns with better interconnectivity than scaffolds from the other two ratios. Higher dioxane-water ratios resulted in a larger contact angle and thus less wettability for the fabricated scaffold, while scaffolds fabricated from higher concentrations of P(PF-co-CL) or high dioxane-water ratios had better biomineralization after soaking in simulated body fluid. In vitro cell viability testing showed the scaffolds had good biocompatibility with both bone and nerve cells. The results indicate that the polymer concentration and solvents ratio significantly affect the formation of porous structures, and optimum processing parameters were found to be 8{\%} polymer concentration and 22{\%} to 24{\%} water content. These porous P(PF-co-CL) scaffolds fabricated via TIPS may be useful in various tissue engineering applications",
keywords = "P(PF-co-CL), porous scaffold, thermally induced phase separation, tissue engineering",
author = "Ji Guo and Xifeng Liu and {Lee Miller}, A. and Waletzki, {Brian E.} and Yaszemski, {Michael J} and Lichun Lu",
year = "2017",
month = "1",
day = "1",
doi = "10.1002/jbm.a.35862",
language = "English (US)",
volume = "105",
pages = "226--235",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Novel porous poly(propylene fumarate-co-caprolactone) scaffolds fabricated by thermally induced phase separation

AU - Guo, Ji

AU - Liu, Xifeng

AU - Lee Miller, A.

AU - Waletzki, Brian E.

AU - Yaszemski, Michael J

AU - Lu, Lichun

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Scaffolds with porous structures are highly applicable for tissue engineering and regenerative medicine. In the present study, 3-dimensional poly(propylene fumarate-co-caprolactone) [P(PF-co-CL)] scaffolds were fabricated from a P(PF-co-CL)–dioxane-water ternary system through thermally induced phase separation (TIPS). Cloud points of P(PF-co-CL) in dioxane-water solutions increased with increased solute concentration, but increased dioxane composition decreased cloud point. Among 3 polymer concentrations (4, 8, and 12 wt%), 8 wt% P(PF-co-CL) scaffolds exhibited the best pore interconnectivity, with large, regular sized pores. Scaffolds were formed in 3 solutions with different dioxane-water ratios (74/26, 78/22, and 82/18 wt/wt); the 78/22 wt/wt scaffold had finger-shaped patterns with better interconnectivity than scaffolds from the other two ratios. Higher dioxane-water ratios resulted in a larger contact angle and thus less wettability for the fabricated scaffold, while scaffolds fabricated from higher concentrations of P(PF-co-CL) or high dioxane-water ratios had better biomineralization after soaking in simulated body fluid. In vitro cell viability testing showed the scaffolds had good biocompatibility with both bone and nerve cells. The results indicate that the polymer concentration and solvents ratio significantly affect the formation of porous structures, and optimum processing parameters were found to be 8% polymer concentration and 22% to 24% water content. These porous P(PF-co-CL) scaffolds fabricated via TIPS may be useful in various tissue engineering applications

AB - Scaffolds with porous structures are highly applicable for tissue engineering and regenerative medicine. In the present study, 3-dimensional poly(propylene fumarate-co-caprolactone) [P(PF-co-CL)] scaffolds were fabricated from a P(PF-co-CL)–dioxane-water ternary system through thermally induced phase separation (TIPS). Cloud points of P(PF-co-CL) in dioxane-water solutions increased with increased solute concentration, but increased dioxane composition decreased cloud point. Among 3 polymer concentrations (4, 8, and 12 wt%), 8 wt% P(PF-co-CL) scaffolds exhibited the best pore interconnectivity, with large, regular sized pores. Scaffolds were formed in 3 solutions with different dioxane-water ratios (74/26, 78/22, and 82/18 wt/wt); the 78/22 wt/wt scaffold had finger-shaped patterns with better interconnectivity than scaffolds from the other two ratios. Higher dioxane-water ratios resulted in a larger contact angle and thus less wettability for the fabricated scaffold, while scaffolds fabricated from higher concentrations of P(PF-co-CL) or high dioxane-water ratios had better biomineralization after soaking in simulated body fluid. In vitro cell viability testing showed the scaffolds had good biocompatibility with both bone and nerve cells. The results indicate that the polymer concentration and solvents ratio significantly affect the formation of porous structures, and optimum processing parameters were found to be 8% polymer concentration and 22% to 24% water content. These porous P(PF-co-CL) scaffolds fabricated via TIPS may be useful in various tissue engineering applications

KW - P(PF-co-CL)

KW - porous scaffold

KW - thermally induced phase separation

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84991463641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991463641&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.35862

DO - 10.1002/jbm.a.35862

M3 - Article

C2 - 27513282

AN - SCOPUS:84991463641

VL - 105

SP - 226

EP - 235

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 1

ER -