Novel mutation in the CMV UL97 gene associated with resistance to ganciclovir therapy

Julio C Mendez, Irene Gaw Sia, K. R. Tau, M. J. Espy, T. F. Smith, S. Chou, C. V. Paya

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cytomegalovirus (CMV) strains resistant to ganciclovir have been associated with specific mutations in the UL97 and UL54 genes. The UL97 gene of a CMV strain isolated from a renal transplant recipient before and after 438 days of ganciclovir treatment was amplified by polymerase chain reaction and sequenced. A novel mutation resulting in deletion of codons 595 to 603 was identified in the viral DNA from specimens obtained after, but not before, prolonged ganciclovir therapy. Clinical and virological resolution of CMV disease occurred after switching to foscarnet therapy. Although many ganciclovir resistance mutations have been mapped to the UL97 codon range 591-607, this one is unusual in that it involves deletion of half these codons. Because UL97 seems to be necessary for effective CMV replication, this deletion suggests that much of codons 591-607 can be removed without destroying the biological function of UL97, and that this codon range can be altered in various ways to affect ganciclovir susceptibility. Rapid, flexible genotypic assays directed at this part of UL97 may facilitate the early recognition of ganciclovir resistance.

Original languageEnglish (US)
Pages (from-to)755-757
Number of pages3
JournalTransplantation
Volume67
Issue number5
StatePublished - Mar 15 1999
Externally publishedYes

Fingerprint

Ganciclovir
Cytomegalovirus
Codon
Mutation
Genes
Therapeutics
Foscarnet
Viral DNA
Kidney
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Mendez, J. C., Sia, I. G., Tau, K. R., Espy, M. J., Smith, T. F., Chou, S., & Paya, C. V. (1999). Novel mutation in the CMV UL97 gene associated with resistance to ganciclovir therapy. Transplantation, 67(5), 755-757.

Novel mutation in the CMV UL97 gene associated with resistance to ganciclovir therapy. / Mendez, Julio C; Sia, Irene Gaw; Tau, K. R.; Espy, M. J.; Smith, T. F.; Chou, S.; Paya, C. V.

In: Transplantation, Vol. 67, No. 5, 15.03.1999, p. 755-757.

Research output: Contribution to journalArticle

Mendez, JC, Sia, IG, Tau, KR, Espy, MJ, Smith, TF, Chou, S & Paya, CV 1999, 'Novel mutation in the CMV UL97 gene associated with resistance to ganciclovir therapy', Transplantation, vol. 67, no. 5, pp. 755-757.
Mendez, Julio C ; Sia, Irene Gaw ; Tau, K. R. ; Espy, M. J. ; Smith, T. F. ; Chou, S. ; Paya, C. V. / Novel mutation in the CMV UL97 gene associated with resistance to ganciclovir therapy. In: Transplantation. 1999 ; Vol. 67, No. 5. pp. 755-757.
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AU - Chou, S.

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