Novel candidate tumor marker genes for lung adenocarcinoma

Kan Kan Wang, Ni Liu, Nikolina Radulovich, Dennis A. Wigle, Michael R. Johnston, Frances A. Shepherd, Mark D. Minden, Ming Sound Tsao

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Using the representational difference analysis (RDA) technique on pooled mRNA of five primary lung adenocarcinomas and their corresponding non-neoplastic lung tissues, we identified six genes that were putatively overexpressed in this type of lung cancer. Five corresponded to previously isolated genes, while one (Lc19) matched with the sequence of an unannotated EST. Real-time RT-PCR analyses of expression levels in a panel of 34 paired primary non-small cell lung cancer (NSCLC) and corresponding grossly normal appearing lung tissues confirmed the common overexpression of these genes in non-small cell lung cancer. Among these genes, overexpression of Lc19, hyaluronan binding protein 2 (HABP2) and crystalline-mu appeared more specific to adenocarcinoma, whereas ceruloplasmin, integrin α-11 and collagen type XI alpha 1 were overexpressed at high frequency among both adenocarcinoma and squamous cell carcinoma. These genes represent novel candidate tumor biomarker genes for NSCLC and its histological subtypes.

Original languageEnglish (US)
Pages (from-to)7598-7604
Number of pages7
JournalOncogene
Volume21
Issue number49
DOIs
StatePublished - 2002

Keywords

  • Biomarker
  • Differential expression
  • Lung cancer
  • Microarray
  • Subtractive hybridization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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