Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish

Ang Samden Sherpa; Daniel Schossow; Michael Lenning; Paul Marsh; Nick Garzon; Peter Hofsteen; Hung Cao; Jingchun Yang; Xiaolei Xu; Van Nguyen Thi Thanh; Chi Tran Nhu; Tung Thanh Bui; Trinh Chu Duc

doi:10.1109/ICSENS.2017.8234354

Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish

Ang Samden Sherpa, Daniel Schossow, Michael Lenning, Paul Marsh, Nick Garzon, Peter Hofsteen, Hung Cao, Jingchun Yang, Xiaolei Xu, Van Nguyen Thi Thanh, Chi Tran Nhu, Tung Thanh Bui, Trinh Chu Duc

Biochemistry and Molecular Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

This paper reports polymer-based apparatus with embedded flexible thin-film electrodes to monitor electrocardiogram (ECG) of zebrafish under mild or zero anesthesia. The apparatus were made of polydimethylsiloxane (PDMS) using the molding technique with molds formed by 3D printing. The system is capable of acquiring intrinsic ECG from multiple fish simultaneously, thus introducing a novel method to phenotypically assess the hearts' functionalities, supporting heart regeneration studies, drug screening and other related bioinvestigations using the zebrafish model. Smart algorithms were developed to facilitate data processing and analyses. The recorded ECG was compared with that of the same fish under full sedation and a proof-of-concept 4-chamber system was demonstrated. The process does not require a cleanroom, therefore being cost effective and holding promise to accelerate numerous studies in various disciplines using the zebrafish model.

Original language	English (US)
Title of host publication	IEEE SENSORS 2017 - Conference Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1-3
Number of pages	3
ISBN (Electronic)	9781509010127
DOIs	https://doi.org/10.1109/ICSENS.2017.8234354
State	Published - Dec 21 2017
Event	16th IEEE SENSORS Conference, ICSENS 2017 - Glasgow, United Kingdom Duration: Oct 30 2017 → Nov 1 2017

Publication series

Name	Proceedings of IEEE Sensors
Volume	2017-December
ISSN (Print)	1930-0395
ISSN (Electronic)	2168-9229

Other

Other	16th IEEE SENSORS Conference, ICSENS 2017
Country/Territory	United Kingdom
City	Glasgow
Period	10/30/17 → 11/1/17

Keywords

Biological Investigations
Electrocardiogram (ECG)
Heart Diseases
Phenotype screening
Zebrafish

ASJC Scopus subject areas

Electrical and Electronic Engineering

Access to Document

10.1109/ICSENS.2017.8234354

Cite this

Sherpa, A. S., Schossow, D., Lenning, M., Marsh, P., Garzon, N., Hofsteen, P., Cao, H., Yang, J., Xu, X., Thanh, V. N. T., Nhu, C. T., Bui, T. T., & Duc, T. C. (2017). Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish. In IEEE SENSORS 2017 - Conference Proceedings (pp. 1-3). (Proceedings of IEEE Sensors; Vol. 2017-December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2017.8234354

Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish. / Sherpa, Ang Samden; Schossow, Daniel; Lenning, Michael et al.
IEEE SENSORS 2017 - Conference Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. p. 1-3 (Proceedings of IEEE Sensors; Vol. 2017-December).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sherpa, AS, Schossow, D, Lenning, M, Marsh, P, Garzon, N, Hofsteen, P, Cao, H, Yang, J, Xu, X, Thanh, VNT, Nhu, CT, Bui, TT & Duc, TC 2017, Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish. in IEEE SENSORS 2017 - Conference Proceedings. Proceedings of IEEE Sensors, vol. 2017-December, Institute of Electrical and Electronics Engineers Inc., pp. 1-3, 16th IEEE SENSORS Conference, ICSENS 2017, Glasgow, United Kingdom, 10/30/17. https://doi.org/10.1109/ICSENS.2017.8234354

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abstract = "This paper reports polymer-based apparatus with embedded flexible thin-film electrodes to monitor electrocardiogram (ECG) of zebrafish under mild or zero anesthesia. The apparatus were made of polydimethylsiloxane (PDMS) using the molding technique with molds formed by 3D printing. The system is capable of acquiring intrinsic ECG from multiple fish simultaneously, thus introducing a novel method to phenotypically assess the hearts' functionalities, supporting heart regeneration studies, drug screening and other related bioinvestigations using the zebrafish model. Smart algorithms were developed to facilitate data processing and analyses. The recorded ECG was compared with that of the same fish under full sedation and a proof-of-concept 4-chamber system was demonstrated. The process does not require a cleanroom, therefore being cost effective and holding promise to accelerate numerous studies in various disciplines using the zebrafish model.",

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N2 - This paper reports polymer-based apparatus with embedded flexible thin-film electrodes to monitor electrocardiogram (ECG) of zebrafish under mild or zero anesthesia. The apparatus were made of polydimethylsiloxane (PDMS) using the molding technique with molds formed by 3D printing. The system is capable of acquiring intrinsic ECG from multiple fish simultaneously, thus introducing a novel method to phenotypically assess the hearts' functionalities, supporting heart regeneration studies, drug screening and other related bioinvestigations using the zebrafish model. Smart algorithms were developed to facilitate data processing and analyses. The recorded ECG was compared with that of the same fish under full sedation and a proof-of-concept 4-chamber system was demonstrated. The process does not require a cleanroom, therefore being cost effective and holding promise to accelerate numerous studies in various disciplines using the zebrafish model.

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Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish

Abstract

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Keywords

ASJC Scopus subject areas

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