Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish

Ang Samden Sherpa, Daniel Schossow, Michael Lenning, Paul Marsh, Nick Garzon, Peter Hofsteen, Hung Cao, Jingchun Yang, Xiaolei Xu, Van Nguyen Thi Thanh, Chi Tran Nhu, Tung Thanh Bui, Trinh Chu Duc

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

This paper reports polymer-based apparatus with embedded flexible thin-film electrodes to monitor electrocardiogram (ECG) of zebrafish under mild or zero anesthesia. The apparatus were made of polydimethylsiloxane (PDMS) using the molding technique with molds formed by 3D printing. The system is capable of acquiring intrinsic ECG from multiple fish simultaneously, thus introducing a novel method to phenotypically assess the hearts' functionalities, supporting heart regeneration studies, drug screening and other related bioinvestigations using the zebrafish model. Smart algorithms were developed to facilitate data processing and analyses. The recorded ECG was compared with that of the same fish under full sedation and a proof-of-concept 4-chamber system was demonstrated. The process does not require a cleanroom, therefore being cost effective and holding promise to accelerate numerous studies in various disciplines using the zebrafish model.

Original languageEnglish (US)
Title of host publicationIEEE SENSORS 2017 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-3
Number of pages3
ISBN (Electronic)9781509010127
DOIs
StatePublished - Dec 21 2017
Event16th IEEE SENSORS Conference, ICSENS 2017 - Glasgow, United Kingdom
Duration: Oct 30 2017Nov 1 2017

Publication series

NameProceedings of IEEE Sensors
Volume2017-December
ISSN (Print)1930-0395
ISSN (Electronic)2168-9229

Other

Other16th IEEE SENSORS Conference, ICSENS 2017
CountryUnited Kingdom
CityGlasgow
Period10/30/1711/1/17

Keywords

  • Biological Investigations
  • Electrocardiogram (ECG)
  • Heart Diseases
  • Phenotype screening
  • Zebrafish

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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  • Cite this

    Sherpa, A. S., Schossow, D., Lenning, M., Marsh, P., Garzon, N., Hofsteen, P., Cao, H., Yang, J., Xu, X., Thanh, V. N. T., Nhu, C. T., Bui, T. T., & Duc, T. C. (2017). Novel apparatus for simultaneous monitoring of electrocardiogram in awake zebrafish. In IEEE SENSORS 2017 - Conference Proceedings (pp. 1-3). (Proceedings of IEEE Sensors; Vol. 2017-December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2017.8234354