Novel adenomatous polyposis coli gene promoter is located 40 kb upstream of the initiating methionine

N. Karagianni; M. C. Ly; S. Psarras; K. Chlichlia; V. Schirrmacher; F. Gounari; K. Khazaie

doi:10.1016/j.ygeno.2004.09.005

Novel adenomatous polyposis coli gene promoter is located 40 kb upstream of the initiating methionine

N. Karagianni, M. C. Ly, S. Psarras, K. Chlichlia, V. Schirrmacher, F. Gounari, K. Khazaie

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The product of the oncosuppressor adenomatous polyposis coli (APC) gene is involved in cell cycle arrest and apoptosis and its loss of function is associated with the development of colorectal carcinogenesis. Its transcriptional regulation seems rather complex and has not been completely elucidated up to now. In an attempt to identify the transcription start sites for the mouse Apc gene we have detected a novel transcript in mouse embryonic stem (ES) cells and colon tissue. This transcript contains an untranslated exon, whose flanking sequences exhibited strong promoter activity in transient transfection experiments. These results suggest that we have identified a novel promoter for the mouse Apc gene, localized about 40 kb upstream of the initiating methionine, which drives expression of the unique Apc transcript type detected in undifferentiated totipotent ES cells. Transcripts bearing the novel exon combined either with exon 1 or with exon 2 were detected in all mouse tissues tested.

Original language	English (US)
Pages (from-to)	231-237
Number of pages	7
Journal	Genomics
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/j.ygeno.2004.09.005
State	Published - Feb 2005

Keywords

5′-RACE
Adenomatous polyposis coli
Promoter
Totipotent stem cells
Untranslated exon

ASJC Scopus subject areas

Genetics

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10.1016/j.ygeno.2004.09.005

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title = "Novel adenomatous polyposis coli gene promoter is located 40 kb upstream of the initiating methionine",

abstract = "The product of the oncosuppressor adenomatous polyposis coli (APC) gene is involved in cell cycle arrest and apoptosis and its loss of function is associated with the development of colorectal carcinogenesis. Its transcriptional regulation seems rather complex and has not been completely elucidated up to now. In an attempt to identify the transcription start sites for the mouse Apc gene we have detected a novel transcript in mouse embryonic stem (ES) cells and colon tissue. This transcript contains an untranslated exon, whose flanking sequences exhibited strong promoter activity in transient transfection experiments. These results suggest that we have identified a novel promoter for the mouse Apc gene, localized about 40 kb upstream of the initiating methionine, which drives expression of the unique Apc transcript type detected in undifferentiated totipotent ES cells. Transcripts bearing the novel exon combined either with exon 1 or with exon 2 were detected in all mouse tissues tested.",

keywords = "5′-RACE, Adenomatous polyposis coli, Promoter, Totipotent stem cells, Untranslated exon",

author = "N. Karagianni and Ly, {M. C.} and S. Psarras and K. Chlichlia and V. Schirrmacher and F. Gounari and K. Khazaie",

note = "Funding Information: We thank Wolf Herman Friedman for his interest and support. Min Li Weber (DKFZ, Heidelberg) is kindly acknowledged for her advice and encouragement. Niki Karagianni was a recipient of an IARC (WHO) fellowship. Stelios Psarras was supported by a post-vert INSERM fellowship. Annette Lichtenauer is acknowledged for excellent technical support. This work was supported by ARC, France, and by Department of Defense Idea Award DAMD PC020746.",

year = "2005",

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doi = "10.1016/j.ygeno.2004.09.005",

language = "English (US)",

volume = "85",

pages = "231--237",

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AU - Ly, M. C.

AU - Psarras, S.

AU - Chlichlia, K.

AU - Schirrmacher, V.

AU - Gounari, F.

AU - Khazaie, K.

N1 - Funding Information: We thank Wolf Herman Friedman for his interest and support. Min Li Weber (DKFZ, Heidelberg) is kindly acknowledged for her advice and encouragement. Niki Karagianni was a recipient of an IARC (WHO) fellowship. Stelios Psarras was supported by a post-vert INSERM fellowship. Annette Lichtenauer is acknowledged for excellent technical support. This work was supported by ARC, France, and by Department of Defense Idea Award DAMD PC020746.

PY - 2005/2

Y1 - 2005/2

N2 - The product of the oncosuppressor adenomatous polyposis coli (APC) gene is involved in cell cycle arrest and apoptosis and its loss of function is associated with the development of colorectal carcinogenesis. Its transcriptional regulation seems rather complex and has not been completely elucidated up to now. In an attempt to identify the transcription start sites for the mouse Apc gene we have detected a novel transcript in mouse embryonic stem (ES) cells and colon tissue. This transcript contains an untranslated exon, whose flanking sequences exhibited strong promoter activity in transient transfection experiments. These results suggest that we have identified a novel promoter for the mouse Apc gene, localized about 40 kb upstream of the initiating methionine, which drives expression of the unique Apc transcript type detected in undifferentiated totipotent ES cells. Transcripts bearing the novel exon combined either with exon 1 or with exon 2 were detected in all mouse tissues tested.

AB - The product of the oncosuppressor adenomatous polyposis coli (APC) gene is involved in cell cycle arrest and apoptosis and its loss of function is associated with the development of colorectal carcinogenesis. Its transcriptional regulation seems rather complex and has not been completely elucidated up to now. In an attempt to identify the transcription start sites for the mouse Apc gene we have detected a novel transcript in mouse embryonic stem (ES) cells and colon tissue. This transcript contains an untranslated exon, whose flanking sequences exhibited strong promoter activity in transient transfection experiments. These results suggest that we have identified a novel promoter for the mouse Apc gene, localized about 40 kb upstream of the initiating methionine, which drives expression of the unique Apc transcript type detected in undifferentiated totipotent ES cells. Transcripts bearing the novel exon combined either with exon 1 or with exon 2 were detected in all mouse tissues tested.

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KW - Promoter

KW - Totipotent stem cells

KW - Untranslated exon

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Novel adenomatous polyposis coli gene promoter is located 40 kb upstream of the initiating methionine

Abstract

Keywords

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