Normal FHIT transcripts in renal cell cancer- and lung cancer-derived cell lines, including a cell line with a homozygous deletion in the FRA3G region

Anke Van Den Berg, Tineke G. Draaijers, Klaas Kok, Tineke Timmer, Anneke Y. Van Der Veen, Patrick M J F Veldhuis, Lou De Leij, Claus D. Gerhartz, Susan L. Naylor, David I Smith, Charles H C M Buys

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The recently identified FHIT gene encompasses the FRA3B region and the breakpoint of a constitutive t(3;8) occurring in a family with hereditary renal cell cancer. Occurrence of aberrant transcripts in different types of turnouts has led to the suggestion that FHIT might play a critical role in the development of various types of cancer. We have analyzed the gene and its transcripts in lung cancers and renal cell cancer-derived cell lines. A lung adenocarcinoma cell line, GLC-A2, appeared to have a homozygous deletion in intron 5 of FHIT. RT-PCR analysis revealed a normal-sized PCR product in all of the cell lines, including GLC-A2. A number of them had an additional aberrant product. Analysis of a great number of control cell lines and tissues showed that the majority of these also had aberrant PCR products in addition to a normal-sized PCR product. Different specimens of the same cell type showed variable additional RT-PCR products. Normal-sized PCR products had a sequence identical to the FHIT sequence. PCR products longer than normal bad insertions of different sizes at different positions. With three exceptions, PCR products shorter than normal represented FHIT sequences missing one or more entire exons. Thus, the presence of aberrant transcripts is not cancer-specific. Conceivably, sequences responsible for the instability of the FRA3B region are being transcribed into FHIT pre-mRNA and may cause the abnormal splicing and processing of the transcripts.

Original languageEnglish (US)
Pages (from-to)220-227
Number of pages8
JournalGenes Chromosomes and Cancer
Volume19
Issue number4
DOIs
StatePublished - Aug 1997
Externally publishedYes

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Renal Cell Carcinoma
Lung Neoplasms
Cell Line
Polymerase Chain Reaction
varespladib methyl
RNA Precursors
Introns
Genes
Exons
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Normal FHIT transcripts in renal cell cancer- and lung cancer-derived cell lines, including a cell line with a homozygous deletion in the FRA3G region. / Van Den Berg, Anke; Draaijers, Tineke G.; Kok, Klaas; Timmer, Tineke; Van Der Veen, Anneke Y.; Veldhuis, Patrick M J F; De Leij, Lou; Gerhartz, Claus D.; Naylor, Susan L.; Smith, David I; Buys, Charles H C M.

In: Genes Chromosomes and Cancer, Vol. 19, No. 4, 08.1997, p. 220-227.

Research output: Contribution to journalArticle

Van Den Berg, Anke ; Draaijers, Tineke G. ; Kok, Klaas ; Timmer, Tineke ; Van Der Veen, Anneke Y. ; Veldhuis, Patrick M J F ; De Leij, Lou ; Gerhartz, Claus D. ; Naylor, Susan L. ; Smith, David I ; Buys, Charles H C M. / Normal FHIT transcripts in renal cell cancer- and lung cancer-derived cell lines, including a cell line with a homozygous deletion in the FRA3G region. In: Genes Chromosomes and Cancer. 1997 ; Vol. 19, No. 4. pp. 220-227.
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