Normal early pregnancy: A transient state of epigenetic change favoring hypomethylation

Wendy M. White, Brian C. Brost, Zhifu D Sun, Carl Rose, Iasmina Craici, Steven J. Wagner, Stephen T Turner, Vesna D Garovic

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The objective of this study was to analyze genome-wide differential methylation patterns in maternal leukocyte DNA in early pregnant and non-pregnant states. This is an age and body mass index matched case-control study comparing the methylation patterns of 27,578 cytosine-guanine (CpG) sites in 14,495 genes in maternal leukocyte DNA in early pregnancy (n = 14), in the same women postpartum (n = 14), and in nulligravid women (n = 14) on a BeadChip platform. Transient widespread hypomethylation was found in early pregnancy as compared with the non-pregnant states. Methylation of nine genes was significantly different in early pregnancy compared with both postpartum and nulligravid states (<10% False Discovery Rate). Early pregnancy may be characterized by widespread hypomethylation compared with non-pregnant states; there is no apparent permanent methylation imprint after a normal term gestation. Nine potential candidate genes were identified as differentially methylated in early pregnancy and may play a role in the maternal adaptation to pregnancy.

Original languageEnglish (US)
Pages (from-to)729-734
Number of pages6
JournalEpigenetics
Volume7
Issue number7
DOIs
StatePublished - Jul 2012

Fingerprint

Epigenomics
Pregnancy
Methylation
Mothers
Postpartum Period
Leukocytes
Genes
Cytosine
DNA
Guanine
Case-Control Studies
Body Mass Index
Genome

Keywords

  • DNA methylation
  • Epigenetics
  • Immune
  • Leukocyte
  • Maternal
  • Postpartum
  • Pregnancy

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Normal early pregnancy : A transient state of epigenetic change favoring hypomethylation. / White, Wendy M.; Brost, Brian C.; Sun, Zhifu D; Rose, Carl; Craici, Iasmina; Wagner, Steven J.; Turner, Stephen T; Garovic, Vesna D.

In: Epigenetics, Vol. 7, No. 7, 07.2012, p. 729-734.

Research output: Contribution to journalArticle

White, Wendy M. ; Brost, Brian C. ; Sun, Zhifu D ; Rose, Carl ; Craici, Iasmina ; Wagner, Steven J. ; Turner, Stephen T ; Garovic, Vesna D. / Normal early pregnancy : A transient state of epigenetic change favoring hypomethylation. In: Epigenetics. 2012 ; Vol. 7, No. 7. pp. 729-734.
@article{f7eac17ebc1e4cf398dfb2712401af36,
title = "Normal early pregnancy: A transient state of epigenetic change favoring hypomethylation",
abstract = "The objective of this study was to analyze genome-wide differential methylation patterns in maternal leukocyte DNA in early pregnant and non-pregnant states. This is an age and body mass index matched case-control study comparing the methylation patterns of 27,578 cytosine-guanine (CpG) sites in 14,495 genes in maternal leukocyte DNA in early pregnancy (n = 14), in the same women postpartum (n = 14), and in nulligravid women (n = 14) on a BeadChip platform. Transient widespread hypomethylation was found in early pregnancy as compared with the non-pregnant states. Methylation of nine genes was significantly different in early pregnancy compared with both postpartum and nulligravid states (<10{\%} False Discovery Rate). Early pregnancy may be characterized by widespread hypomethylation compared with non-pregnant states; there is no apparent permanent methylation imprint after a normal term gestation. Nine potential candidate genes were identified as differentially methylated in early pregnancy and may play a role in the maternal adaptation to pregnancy.",
keywords = "DNA methylation, Epigenetics, Immune, Leukocyte, Maternal, Postpartum, Pregnancy",
author = "White, {Wendy M.} and Brost, {Brian C.} and Sun, {Zhifu D} and Carl Rose and Iasmina Craici and Wagner, {Steven J.} and Turner, {Stephen T} and Garovic, {Vesna D}",
year = "2012",
month = "7",
doi = "10.4161/epi.20388",
language = "English (US)",
volume = "7",
pages = "729--734",
journal = "Epigenetics",
issn = "1559-2294",
publisher = "Landes Bioscience",
number = "7",

}

TY - JOUR

T1 - Normal early pregnancy

T2 - A transient state of epigenetic change favoring hypomethylation

AU - White, Wendy M.

AU - Brost, Brian C.

AU - Sun, Zhifu D

AU - Rose, Carl

AU - Craici, Iasmina

AU - Wagner, Steven J.

AU - Turner, Stephen T

AU - Garovic, Vesna D

PY - 2012/7

Y1 - 2012/7

N2 - The objective of this study was to analyze genome-wide differential methylation patterns in maternal leukocyte DNA in early pregnant and non-pregnant states. This is an age and body mass index matched case-control study comparing the methylation patterns of 27,578 cytosine-guanine (CpG) sites in 14,495 genes in maternal leukocyte DNA in early pregnancy (n = 14), in the same women postpartum (n = 14), and in nulligravid women (n = 14) on a BeadChip platform. Transient widespread hypomethylation was found in early pregnancy as compared with the non-pregnant states. Methylation of nine genes was significantly different in early pregnancy compared with both postpartum and nulligravid states (<10% False Discovery Rate). Early pregnancy may be characterized by widespread hypomethylation compared with non-pregnant states; there is no apparent permanent methylation imprint after a normal term gestation. Nine potential candidate genes were identified as differentially methylated in early pregnancy and may play a role in the maternal adaptation to pregnancy.

AB - The objective of this study was to analyze genome-wide differential methylation patterns in maternal leukocyte DNA in early pregnant and non-pregnant states. This is an age and body mass index matched case-control study comparing the methylation patterns of 27,578 cytosine-guanine (CpG) sites in 14,495 genes in maternal leukocyte DNA in early pregnancy (n = 14), in the same women postpartum (n = 14), and in nulligravid women (n = 14) on a BeadChip platform. Transient widespread hypomethylation was found in early pregnancy as compared with the non-pregnant states. Methylation of nine genes was significantly different in early pregnancy compared with both postpartum and nulligravid states (<10% False Discovery Rate). Early pregnancy may be characterized by widespread hypomethylation compared with non-pregnant states; there is no apparent permanent methylation imprint after a normal term gestation. Nine potential candidate genes were identified as differentially methylated in early pregnancy and may play a role in the maternal adaptation to pregnancy.

KW - DNA methylation

KW - Epigenetics

KW - Immune

KW - Leukocyte

KW - Maternal

KW - Postpartum

KW - Pregnancy

UR - http://www.scopus.com/inward/record.url?scp=84863809548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863809548&partnerID=8YFLogxK

U2 - 10.4161/epi.20388

DO - 10.4161/epi.20388

M3 - Article

C2 - 22647708

AN - SCOPUS:84863809548

VL - 7

SP - 729

EP - 734

JO - Epigenetics

JF - Epigenetics

SN - 1559-2294

IS - 7

ER -