Norepinephrine spillover in forearm and subcutaneous Adipose tissue before and after eating

J. N. Patel, G. Eisenhofer, S. W. Coppack, J. M. Miles

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The sympathetic nervous system regulates lipolysis. There are regional differences in the sensitivity of lipolysis to adrenergic regulation. Little is known about regional sympathetic activity in response to eating in humans. We studied the effect of feeding on systemic and local sympathetic nervous system activity and lipolysis in lean healthy subjects (three women and five men; age, 27.0 ± 2.0; body mass index, 23.4 ± 1.2 kg/m-2) using isotope dilution methodology and arterio-venous sampling. Feeding increased arterial norepinephrine (NE) concentration (mean premeal, 0.96 ± 0.12 nmol/L·L; mean postmeal, 1.28 ± 0.14 nmol/L·L; P < 0.02) and total body NE spillover (mean premeal, 2.11 ± 0.30 nmol/min·L; mean postmeal, 2.76 ± 0.31 nmol/min·L; P < 0.02), whereas the arterial epinephrine concentration decreased (mean premeal, 289 ± 61 pmol/L; mean postmeal, 170 ± 5 pmol/L; P < 0.02). Palmitate concentration and total body systemic rate of appearance of palmitate declined postprandially (mean premeal, 117 ± 15 μmol/min; mean postmeal, 38 ± 4 μmol/min; P < 0.01). NE spillover increased by the same proportion in both forearm and adipose tissue [in forearm, mean premeal and postmeal, 1.02 ± 0.11 and 2.41 ± 0.44 nmol/100 mL·min, respectively (P < 0.02); in adipose tissue, mean premeal and postmeal, 0.41 ± 0.12 and 0.73 ±0.17 nmol/100 g·min, respectively (P < 0.02)]. The results show that a meal caused differential changes in systemic sympatho-adrenal activity and an increase in sympathetic activity in adipose tissue postprandially, However, this increase in postprandial sympathetic activity was not enough to overcome the inhibition of lipolysis by insulin.

Original languageEnglish (US)
Pages (from-to)2815-2819
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume84
Issue number8
StatePublished - 1999
Externally publishedYes

Fingerprint

Lipolysis
Subcutaneous Fat
Forearm
Norepinephrine
Eating
Tissue
Adipose Tissue
Palmitates
Sympathetic Nervous System
Neurology
Isotopes
Adrenergic Agents
Epinephrine
Dilution
Meals
Healthy Volunteers
Body Mass Index
Insulin
Sampling

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Norepinephrine spillover in forearm and subcutaneous Adipose tissue before and after eating. / Patel, J. N.; Eisenhofer, G.; Coppack, S. W.; Miles, J. M.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 84, No. 8, 1999, p. 2815-2819.

Research output: Contribution to journalArticle

Patel, J. N. ; Eisenhofer, G. ; Coppack, S. W. ; Miles, J. M. / Norepinephrine spillover in forearm and subcutaneous Adipose tissue before and after eating. In: Journal of Clinical Endocrinology and Metabolism. 1999 ; Vol. 84, No. 8. pp. 2815-2819.
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abstract = "The sympathetic nervous system regulates lipolysis. There are regional differences in the sensitivity of lipolysis to adrenergic regulation. Little is known about regional sympathetic activity in response to eating in humans. We studied the effect of feeding on systemic and local sympathetic nervous system activity and lipolysis in lean healthy subjects (three women and five men; age, 27.0 ± 2.0; body mass index, 23.4 ± 1.2 kg/m-2) using isotope dilution methodology and arterio-venous sampling. Feeding increased arterial norepinephrine (NE) concentration (mean premeal, 0.96 ± 0.12 nmol/L·L; mean postmeal, 1.28 ± 0.14 nmol/L·L; P < 0.02) and total body NE spillover (mean premeal, 2.11 ± 0.30 nmol/min·L; mean postmeal, 2.76 ± 0.31 nmol/min·L; P < 0.02), whereas the arterial epinephrine concentration decreased (mean premeal, 289 ± 61 pmol/L; mean postmeal, 170 ± 5 pmol/L; P < 0.02). Palmitate concentration and total body systemic rate of appearance of palmitate declined postprandially (mean premeal, 117 ± 15 μmol/min; mean postmeal, 38 ± 4 μmol/min; P < 0.01). NE spillover increased by the same proportion in both forearm and adipose tissue [in forearm, mean premeal and postmeal, 1.02 ± 0.11 and 2.41 ± 0.44 nmol/100 mL·min, respectively (P < 0.02); in adipose tissue, mean premeal and postmeal, 0.41 ± 0.12 and 0.73 ±0.17 nmol/100 g·min, respectively (P < 0.02)]. The results show that a meal caused differential changes in systemic sympatho-adrenal activity and an increase in sympathetic activity in adipose tissue postprandially, However, this increase in postprandial sympathetic activity was not enough to overcome the inhibition of lipolysis by insulin.",
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