Nonspecific reactions in the hemagglutination inhibition test for detection of rubella antibodies

D. B. Budzko, Diane F Jelinek, B. W. Wilcke

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Approximately 7% of the sera tested to determine the presence of rubella-specific antibodies by the hemagglutination inhibition test demonstrated abnormal patterns of reactivity, rendering the test unreadable. Another 3% of sera were shown to have false-positive titers as high as 1:128. When these abnormally reacting and false-positive sera were heated at 56°C for 30 min after chemical treatment they always converted to negative, indicating the absence of specific rubella hemagglutination-inhibiting antibody. These results were confirmed by fractionation of the sera after sucrose gradient centrifugation. It was established that manifestation of these nonspecific results was dependent on the concentration of Ca2+ or Mn2+. The heat-labile inhibitor(s) responsible for abnormal and false-positive reactions was found not to be complement. This inhibitor(s) was detected in the light fractions of sera and when added to negative sera was capable of reproducing the abnormal patterns of reactivity. These results emphasize the necessity of heating sera for the rubella hemagglutination inhibition test after the chemical removal of nonspecific inhibitors.

Original languageEnglish (US)
Pages (from-to)818-823
Number of pages6
JournalJournal of Clinical Microbiology
Volume13
Issue number5
StatePublished - 1981
Externally publishedYes

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Hemagglutination Inhibition Tests
Serum
Rubella
False Positive Reactions
Hemagglutination
rubella antibodies
Centrifugation
Heating
Sucrose
Hot Temperature
Antibodies

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Nonspecific reactions in the hemagglutination inhibition test for detection of rubella antibodies. / Budzko, D. B.; Jelinek, Diane F; Wilcke, B. W.

In: Journal of Clinical Microbiology, Vol. 13, No. 5, 1981, p. 818-823.

Research output: Contribution to journalArticle

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