Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

Wenqian Hu, Christine Petzold, Jeff Coller, Kristian E. Baker

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

Original languageEnglish (US)
Pages (from-to)244-247
Number of pages4
JournalNature Structural and Molecular Biology
Volume17
Issue number2
DOIs
StatePublished - Feb 1 2010

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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