Noninvolvement of Vβ8+ t cells in murine thyroglobulin-induced experimental autoimmune thyroiditis

B. E. Fuller, A. A. Giraldo, R. W. Motte, G. H. Nabozny, C. S. David, Y. C M Kong

Research output: Contribution to journalArticle

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Abstract

In experimental autoimmune thyroiditis (EAT) induced with mouse thyroglobulin (MTg), T cell receptor (TCR) Vβ gene usage in the pathogenesis of disease is unknown. We report here studies evaluating Vβ8 gene usage in EAT, as Vβ8+ T cells are reportedly involved in some experimental autoimmune diseases. Spleen cells (SC) from MTg-immunized CBA/J (H-2(k) mice were activated in vitro for adoptive transfer into syngeneic recipients. Elimination of Vβ8+ T cells by treating recipients with Vβ8 monoclonal antibody (mAb) following transfer of MTg-activated SC did not reduce disease severity. Conversely, MTg-primed SC were stimulated in vitro with Vβ8 mAb or staphylococcal enterotoxin B, which activates Vβ8+ T cells in CBA/J mice. Neither activated population transferred disease, in contrast to cells activated with MTg. Thus, in MTg-induced EAT, Vβ8+ T cells do not play a major role in pathogenesis.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalCellular Immunology
Volume159
Issue number2
DOIs
StatePublished - 1994

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Autoimmune Thyroiditis
Thyroglobulin
T-Lymphocytes
Spleen
Monoclonal Antibodies
T-Cell Receptor Genes
Inbred CBA Mouse
Adoptive Transfer
Autoimmune Diseases
Population
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Fuller, B. E., Giraldo, A. A., Motte, R. W., Nabozny, G. H., David, C. S., & Kong, Y. C. M. (1994). Noninvolvement of Vβ8+ t cells in murine thyroglobulin-induced experimental autoimmune thyroiditis. Cellular Immunology, 159(2), 315-322. https://doi.org/10.1006/cimm.1994.1317

Noninvolvement of Vβ8+ t cells in murine thyroglobulin-induced experimental autoimmune thyroiditis. / Fuller, B. E.; Giraldo, A. A.; Motte, R. W.; Nabozny, G. H.; David, C. S.; Kong, Y. C M.

In: Cellular Immunology, Vol. 159, No. 2, 1994, p. 315-322.

Research output: Contribution to journalArticle

Fuller, BE, Giraldo, AA, Motte, RW, Nabozny, GH, David, CS & Kong, YCM 1994, 'Noninvolvement of Vβ8+ t cells in murine thyroglobulin-induced experimental autoimmune thyroiditis', Cellular Immunology, vol. 159, no. 2, pp. 315-322. https://doi.org/10.1006/cimm.1994.1317
Fuller, B. E. ; Giraldo, A. A. ; Motte, R. W. ; Nabozny, G. H. ; David, C. S. ; Kong, Y. C M. / Noninvolvement of Vβ8+ t cells in murine thyroglobulin-induced experimental autoimmune thyroiditis. In: Cellular Immunology. 1994 ; Vol. 159, No. 2. pp. 315-322.
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