TY - JOUR
T1 - Noninvasive measurement of concurrent single-kidney perfusion, glomerular filtration, and tubular function
AU - Krier, James D.
AU - Ritman, Erik L.
AU - Bajzer, Zeljko
AU - Romero, J. Carlos
AU - Lerman, Amir
AU - Lerman, Lilach O.
PY - 2001
Y1 - 2001
N2 - To assess the reliability of electron beam computed tomography (EBCT), measurements of single-kidney renal blood flow (RBF), glomerular filtration rate (GFR), and intratubular contrast medium concentration (ITC) of radiographic contrast media were quantified in anesthetized pigs before and after acetylcholine-induced vasodilation and diuresis. EBCT measurements were compared with those obtained with intravascular Doppler and inulin clearance. The capability of EBCT to detect chronic changes in single-kidney function was evaluated in pigs with unilateral renal artery stenosis, and their long-term reproducibility in normal pigs was studied repeatedly at 1-mo intervals. EBCT-RBF (ml/min) correlated with Doppler-RBF as RBFEBCT=45+1.07*RBFDoppler, r=0.81. EBCT-GFR (ml/min) correlated with inulin clearance as GFREBCT=11.7+1.02*GFRinulin, r=0.80. During vasodilation, RBF and GFR increased, whereas ITC decreased along the nephron. In renal artery stenosis, single-kidney GFR decreased linearly with the degree of stenosis, and ITC increased along the nephron, indicating increased fluid reabsorption. EBCT-RBF, GFR, and ITC were similar among repeated measurements. This approach might be invaluable for simultaneous quantification of regional hemodynamics and function in the intact kidneys, in a manner potentially applicable to humans.
AB - To assess the reliability of electron beam computed tomography (EBCT), measurements of single-kidney renal blood flow (RBF), glomerular filtration rate (GFR), and intratubular contrast medium concentration (ITC) of radiographic contrast media were quantified in anesthetized pigs before and after acetylcholine-induced vasodilation and diuresis. EBCT measurements were compared with those obtained with intravascular Doppler and inulin clearance. The capability of EBCT to detect chronic changes in single-kidney function was evaluated in pigs with unilateral renal artery stenosis, and their long-term reproducibility in normal pigs was studied repeatedly at 1-mo intervals. EBCT-RBF (ml/min) correlated with Doppler-RBF as RBFEBCT=45+1.07*RBFDoppler, r=0.81. EBCT-GFR (ml/min) correlated with inulin clearance as GFREBCT=11.7+1.02*GFRinulin, r=0.80. During vasodilation, RBF and GFR increased, whereas ITC decreased along the nephron. In renal artery stenosis, single-kidney GFR decreased linearly with the degree of stenosis, and ITC increased along the nephron, indicating increased fluid reabsorption. EBCT-RBF, GFR, and ITC were similar among repeated measurements. This approach might be invaluable for simultaneous quantification of regional hemodynamics and function in the intact kidneys, in a manner potentially applicable to humans.
KW - Electron beam computed tomography
KW - Glomerular filtration rate
KW - Renal blood flow
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U2 - 10.1152/ajprenal.2001.281.4.f630
DO - 10.1152/ajprenal.2001.281.4.f630
M3 - Article
C2 - 11553509
AN - SCOPUS:0034785375
SN - 1931-857X
VL - 281
SP - F630-F638
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 4 50-4
ER -